Journal of Lipid Research, Vol 38, 12-21, Copyright © 1997 by Lipid Research, Inc.

ARTICLES

ApoA-II/apoA-I molar ratio in the HDL particle influences phospholipid transfer protein-mediated HDL interconversion

PJ Pussinen, M Jauhiainen and C Ehnholm
Department of Biochemistry, National Public Health Institute, Helsinki, Finland.

Pig plasma phospholipid transfer protein (PLTP) facilitates interconversion of both human HDL3 and pig HDL into HDL subpopulations of large and small particles. We recently suggested that there are two essential parts in the conversion mechanism, i.e., the release of apoA- I and phospholipids and the fusion of apoA-I-depleted unstable particles. Based on their mass composition and electrophoretic mobility in agarose gel, the released apoA-I-containing particles are similar to previously described pre beta-HDL particles known as primary acceptors of peripheral cholesterol. The aim of this study was to determine whether the apolipoprotein composition of HDL regulates the PLTP- mediated conversion process. Pig HDL was incubated with increasing amounts of human apoA-II which incorporates into pig HDL with a concomitant release of apoA-I and some phospholipids. These hybrid pig HDL particles formed were isolated by a combination of ultracentrifugation at density 1.21 g/ml and gel filtration. The apoA- II/apoA-I molar ratios in the hybrid HDL particles ranged from 0.2 to 7.6 mol/mol. In the maximally modified HDL apoA-I was totally substituted by apoA-II. These particles were incubated in the presence of purified PLTP and the conversion products were isolated and characterized. Both the formation of large particles and the release of apoA-I were inhibited by increasing concentrations of apoA-II in the HDL particle. The hybrid HDL particles behaved similarly as native pig HDL as acceptors of phospholipid from PC-vesicles in the PLTP-assay. This study suggests that the apoA-II/apoA-I molar ratio in the HDL particle regulates PLTP-mediated HDL interconversion.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				U. C. Broedl, W. Jin, I. V. Fuki, J. S. Millar, and D. J. Rader Endothelial lipase is less effective at influencing HDL metabolism in vivo in mice expressing apoA-II J. Lipid Res., October 1, 2006; 47(10): 2191 - 2197. [Abstract] [Full Text] [PDF]

				J. Ji, G. F. Watts, A. G. Johnson, D. C. Chan, E. M. M. Ooi, K.-A. Rye, A. P. Serone, and P. H. R. Barrett High-Density Lipoprotein (HDL) Transport in the Metabolic Syndrome: Application of a New Model for HDL Particle Kinetics J. Clin. Endocrinol. Metab., March 1, 2006; 91(3): 973 - 979. [Abstract] [Full Text] [PDF]

				N. J. Hime, K. J. Drew, K. Wee, P. J. Barter, and K.-A. Rye Formation of high density lipoproteins containing both apolipoprotein A-I and A-II in the rabbit J. Lipid Res., January 1, 2006; 47(1): 115 - 122. [Abstract] [Full Text] [PDF]

				S. Jayaraman, D. L. Gantz, and O. Gursky Kinetic Stabilization and Fusion of Apolipoprotein A-2:DMPC Disks: Comparison with apoA-1 and apoC-1 Biophys. J., April 1, 2005; 88(4): 2907 - 2918. [Abstract] [Full Text] [PDF]

				J. W. J. Beulens, A. Sierksma, A. van Tol, N. Fournier, T. van Gent, J-L. Paul, and H. F. J. Hendriks Moderate alcohol consumption increases cholesterol efflux mediated by ABCA1 J. Lipid Res., September 1, 2004; 45(9): 1716 - 1723. [Abstract] [Full Text] [PDF]

				J. Boucher, T. A. Ramsamy, S. Braschi, D. Sahoo, T. A-M. Neville, and D. L. Sparks Apolipoprotein A-II regulates HDL stability and affects hepatic lipase association and activity J. Lipid Res., May 1, 2004; 45(5): 849 - 858. [Abstract] [Full Text] [PDF]

				H. M. Colhoun, M.-R. Taskinen, J. D. Otvos, P. van den Berg, J. O'Connor, and A. Van Tol Relationship of Phospholipid Transfer Protein Activity to HDL and Apolipoprotein B-Containing Lipoproteins in Subjects With and Without Type 1 Diabetes Diabetes, November 1, 2002; 51(11): 3300 - 3305. [Abstract] [Full Text] [PDF]

				F. Blanco-Vaca, J. C. Escola-Gil, J. M. Martin-Campos, and J. Julve Role of apoA-II in lipid metabolism and atherosclerosis: advances in the study of an enigmatic protein J. Lipid Res., November 1, 2001; 42(11): 1727 - 1739. [Abstract] [Full Text] [PDF]

				F. M. van 't Hooft, G. Ruotolo, S. Boquist, U. de Faire, G. Eggertsen, and A. Hamsten Human Evidence That the Apolipoprotein A-II Gene Is Implicated in Visceral Fat Accumulation and Metabolism of Triglyceride-Rich Lipoproteins Circulation, September 11, 2001; 104(11): 1223 - 1228. [Abstract] [Full Text] [PDF]

				T. Oka, T. Kujiraoka, M. Ito, T. Egashira, S. Takahashi, M. N. Nanjee, N. E. Miller, J. Metso, V. M. Olkkonen, C. Ehnholm, et al. Distribution of phospholipid transfer protein in human plasma: presence of two forms of phospholipid transfer protein, one catalytically active and the other inactive J. Lipid Res., October 1, 2000; 41(10): 1651 - 1657. [Abstract] [Full Text]

				T. Oka, T. Kujiraoka, M. Ito, M. Nagano, M. Ishihara, T. Iwasaki, T. Egashira, N. E. Miller, and H. Hattori Measurement of Human Plasma Phospholipid Transfer Protein by Sandwich ELISA Clin. Chem., September 1, 2000; 46(9): 1357 - 1364. [Abstract] [Full Text] [PDF]

				M. Jauhiainen, J. Huuskonen, M. Baumann, J. Metso, T. Oka, T. Egashira, H. Hattori, V. M. Olkkonen, and C. Ehnholm Phospholipid transfer protein (PLTP) causes proteolytic cleavage of apolipoprotein A-I J. Lipid Res., April 1, 1999; 40(4): 654 - 664. [Abstract] [Full Text]

				K.-A. Rye, M. Jauhiainen, P. J. Barter, and C. Ehnholm Triglyceride-enrichment of high density lipoproteins enhances their remodelling by phospholipid transfer protein J. Lipid Res., March 1, 1998; 39(3): 613 - 622. [Abstract] [Full Text]

				P. J. Pussinen, M. Jauhiainen, J. Metso, L. E. Pyle, Y. L. Marcel, N. H. Fidge, and C. Ehnholm Binding of phospholipid transfer protein (PLTP) to apolipoproteins A-I and A-II: location of a PLTP binding domain in the amino terminal region of apoA-I J. Lipid Res., January 1, 1998; 39(1): 152 - 161. [Abstract] [Full Text]

				D. M. Durbin and A. Jonas The Effect of Apolipoprotein A-II on the Structure and Function of Apolipoprotein A-I in a Homogeneous Reconstituted High Density Lipoprotein Particle J. Biol. Chem., December 12, 1997; 272(50): 31333 - 31339. [Abstract] [Full Text] [PDF]