| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Lipid Research, Vol 38, 35-48, Copyright © 1997 by Lipid Research, Inc.
ARTICLES |
L Krimbou, M Tremblay, J Davignon and JS Cohn
Hyperlipidemia and Atherosclerosis Research Group, Clinical Research Institute of Montreal, Quebec, Canada.
We have used two-dimensional gel electrophoresis to separate and characterize human plasma apolipoprotein (apo) E-containing lipoproteins in the high density lipoprotein (HDL) size range. Lipoproteins were separated from whole plasma by electrophoresis (according to charge) in a 0.75% agarose gel, and then in the second dimension (according to size) in a 2-15% non-denaturing polyacrylamide gradient gel. ApoE-containing lipoproteins were detected by radiography after electrotransfer of lipoproteins to nitrocellulose membranes and incubation with 125I-labeled affinity-purified polyclonal apoE antibody. ApoE-containing lipoproteins in the HDL size range had a particle size ranging from 9 to 18.5 nm in diameter and could be characterized as having either gamma, pre-beta1-, pre-beta2- or alpha- electrophoretic mobility (designated gamma-LpE, pre-beta1-LpE, pre- beta2LpE, and alpha-LpE respectively). gamma-LpE and a substantial proportion of pre-beta1- and pre-beta2-LpE did not co-migrate with apoA- I, apoA-II, apoC-III, or apoB-100. Subsequent experiments focused on the pre-beta1-LpE, pre-beta2LpE, and alpha-LpE subfractions, which represented > 95% of apoE in HDL-sized lipoproteins. Storage of plasma at 4 degrees C or in vitro incubation of plasma at 37 degrees C caused a relative decrease in pre-beta1-LpE and increase in alpha-LpE. Normolipidemic patients with an apoE 2/2 phenotype tended to have increased levels of alpha-LpE, whereas apoE 4/4 subjects tended to have a greater proportion of HDL-apoE as pre-beta1-LpE. Decrease in plasma HDL apoE concentration after an oral fat load was associated with a reduction in the plasma concentration of all HDL-apoE subfractions. These results demonstrate that: 1) apoE-containing HDL are heterogeneous in size and charge; 2) pre-beta1-LpE is a relatively labile HDL subfraction; 3) HDL-apoE subfraction distribution is dependent on apoE phenotype; and 4) all apoE-containing HDL subfractions participate in the plasma transfer of apoE during the postprandial period.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  N. Settasatian, P. J. Barter, and K.-A. Rye Remodeling of apolipoprotein E-containing spherical reconstituted high density lipoproteins by phospholipid transfer protein J. Lipid Res., January 1, 2008; 49(1): 115 - 126. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Guerin, W. Le Goff, E. Duchene, Z. Julia, T. Nguyen, T. Thuren, C. L. Shear, and M. J. Chapman Inhibition of CETP by Torcetrapib Attenuates the Atherogenicity of Postprandial TG-Rich Lipoproteins in Type IIB Hyperlipidemia Arterioscler. Thromb. Vasc. Biol., January 1, 2008; 28(1): 148 - 154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Skropeta, C. Settasatian, M. R. McMahon, K. Shearston, D. Caiazza, K. C. McGrath, W. Jin, D. J. Rader, P. J. Barter, and K.-A. Rye N-Glycosylation regulates endothelial lipase-mediated phospholipid hydrolysis in apoE- and apoA-I-containing high density lipoproteins J. Lipid Res., September 1, 2007; 48(9): 2047 - 2057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-A. Rye, R. Bright, M. Psaltis, and P. J. Barter Regulation of reconstituted high density lipoprotein structure and remodeling by apolipoprotein E J. Lipid Res., May 1, 2006; 47(5): 1025 - 1036. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. L. Ruel, P. Couture, J. S. Cohn, A. Bensadoun, M. Marcil, and B. Lamarche Evidence that hepatic lipase deficiency in humans is not associated with proatherogenic changes in HDL composition and metabolism J. Lipid Res., August 1, 2004; 45(8): 1528 - 1537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Krimbou, M. Denis, B. Haidar, M. Carrier, M. Marcil, and J. Genest Jr. Molecular interactions between apoE and ABCA1: impact on apoE lipidation J. Lipid Res., May 1, 2004; 45(5): 839 - 848. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Cohn, R. Batal, M. Tremblay, H. Jacques, L. Veilleux, C. Rodriguez, O. Mamer, and J. Davignon Plasma turnover of HDL apoC-I, apoC-III, and apoE in humans: in vivo evidence for a link between HDL apoC-III and apoA-I metabolism J. Lipid Res., October 1, 2003; 44(10): 1976 - 1983. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Krimbou, M. Marcil, H. Chiba, and J. Genest Jr. Structural and functional properties of human plasma high density-sized lipoprotein containing only apoE particles J. Lipid Res., May 1, 2003; 44(5): 884 - 892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Shi, X. Wang, K. Tangchitpiyanond, J. Wong, Y. Shi, and A. J. Lusis Atherosclerosis in C3H/HeJ Mice Reconstituted With Apolipoprotein E-Null Bone Marrow Arterioscler. Thromb. Vasc. Biol., April 1, 2002; 22(4): 650 - 655. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Harris, I. R. Graham, S. Schepelmann, A. K. Stannard, M. L. Roberts, B. L. Hodges, V. Hill, A. Amalfitano, D. G. Hassall, J. S. Owen, et al. Acute regression of advanced and retardation of early aortic atheroma in immunocompetent apolipoprotein-E (apoE) deficient mice by administration of a second generation [E1-, E3-, polymerase-] adenovirus vector expressing human apoE Hum. Mol. Genet., January 1, 2002; 11(1): 43 - 58. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Barlage, D. Fröhlich, A. Böttcher, M. Jauhiainen, H. P. Müller, F. Noetzel, G. Rothe, C. Schütt, R. P. Linke, K. J. Lackner, et al. ApoE-containing high density lipoproteins and phospholipid transfer protein activity increase in patients with a systemic inflammatory response J. Lipid Res., February 1, 2001; 42(2): 281 - 290. [Abstract] [Full Text]
|
|
|
|
|
|
T. Athanasopoulos, J. S. Owen, D. Hassall, M. G. Dunckley, J. Drew, J. Goodman, A. D. Tagalakis, D. R. Riddell, and G. Dickson Intramuscular injection of a plasmid vector expressing human apolipoprotein E limits progression of xanthoma and aortic atheroma in apoE-deficient mice Hum. Mol. Genet., October 1, 2000; 9(17): 2545 - 2551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Böttcher, J. Schlosser, F. Kronenberg, H. Dieplinger, G. Knipping, K. J. Lackner, and G. Schmitz Preparative free-solution isotachophoresis for separation of human plasma lipoproteins: apolipoprotein and lipid composition of HDL subfractions J. Lipid Res., June 1, 2000; 41(6): 905 - 915. [Abstract] [Full Text]
|
|
|
|
|
|
A. Schlenck, B. Herbeth, G. Siest, and S. Visvikis Characterization and quantification of serum lipoprotein subfractions by capillary isotachophoresis: relationships with lipid, apolipoprotein, and lipoprotein levels J. Lipid Res., November 1, 1999; 40(11): 2125 - 2133. [Abstract] [Full Text]
|
|
|
|
|
|
K. Tsukamoto, R. Tangirala, S. H. Chun, E. Pure, and D. J. Rader Rapid Regression of Atherosclerosis Induced by Liver-Directed Gene Transfer of ApoE in ApoE-Deficient Mice Arterioscler. Thromb. Vasc. Biol., September 1, 1999; 19(9): 2162 - 2170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Marcoux, M. Tremblay, K. Nakajima, J. Davignon, and J. S. Cohn Characterization of remnant-like particles isolated by immunoaffinity gel from the plasma of type III and type IV hyperlipoproteinemic patients J. Lipid Res., April 1, 1999; 40(4): 636 - 647. [Abstract] [Full Text]
|
|
|
|
|
|
M. Marcil, L. Yu, L. Krimbou, B. Boucher, J. F. Oram, J. S. Cohn, and J. Genest Jr Cellular Cholesterol Transport and Efflux in Fibroblasts Are Abnormal in Subjects With Familial HDL Deficiency Arterioscler. Thromb. Vasc. Biol., January 1, 1999; 19(1): 159 - 169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Krimbou, M. Tremblay, J. Davignon, and J. S. Cohn Association of apolipoprotein E with {alpha}2-macroglobulin in human plasma J. Lipid Res., December 1, 1998; 39(12): 2373 - 2386. [Abstract] [Full Text]
|
|
|
|
 |
|
 Y. Zhu, S. Bellosta, C. Langer, F. Bernini, R. E. Pitas, R. W. Mahley, G. Assmann, and A. von Eckardstein Low-dose expression of a human apolipoprotein E transgene in macrophages restores cholesterol efflux capacity of apolipoprotein E-deficient mouse plasma PNAS, June 23, 1998; 95(13): 7585 - 7590. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Lamarche, K. D. Uffelman, G. Steiner, P. H. R. Barrett, and G. F. Lewis Analysis of particle size and lipid composition as determinants of the metabolic clearance of human high density lipoproteins in a rabbit model J. Lipid Res., June 1, 1998; 39(6): 1162 - 1172. [Abstract] [Full Text]
|
|
|
|
|
|
L. Krimbou, M. Tremblay, H. Jacques, J. Davignon, and J. S. Cohn In vitro factors affecting the concentration of gamma-LpE ({gamma}-LpE) in human plasma J. Lipid Res., April 1, 1998; 39(4): 861 - 872. [Abstract] [Full Text]
|
|
|
|
|
|
R. Batal, M. Tremblay, L. Krimbou, O. Mamer, J. Davignon, J. Genest Jr, and J. S. Cohn Familial HDL Deficiency Characterized by Hypercatabolism of Mature ApoA-I but Not ProApoA-I Arterioscler. Thromb. Vasc. Biol., April 1, 1998; 18(4): 655 - 664. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. K. Tangirala, D. Pratico, G. A. FitzGerald, S. Chun, K. Tsukamoto, C. Maugeais, D. C. Usher, E. Pure, and D. J. Rader Reduction of Isoprostanes and Regression of Advanced Atherosclerosis by Apolipoprotein E J. Biol. Chem., January 5, 2001; 276(1): 261 - 266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Krimbou, M. Marcil, J. Davignon, and J. Genest Jr. Interaction of Lecithin:Cholesterol Acyltransferase (LCAT){middle dot}alpha 2-Macroglobulin Complex with Low Density Lipoprotein Receptor-related Protein (LRP). EVIDENCE FOR AN alpha 2-MACROGLOBULIN/LRP RECEPTOR-MEDIATED SYSTEM PARTICIPATING IN LCAT CLEARANCE J. Biol. Chem., August 24, 2001; 276(35): 33241 - 33248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Shi, X. Wang, K. Tangchitpiyanond, J. Wong, Y. Shi, and A. J. Lusis Atherosclerosis in C3H/HeJ Mice Reconstituted With Apolipoprotein E-Null Bone Marrow Arterioscler. Thromb. Vasc. Biol., April 1, 2002; 22(4): 650 - 655. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
