Adipose differentiation-related protein is an ubiquitously expressed lipid storage droplet-associated protein

Adipose differentiation-related protein is an ubiquitously expressed lipid storage droplet-associated protein

DL Brasaemle, T Barber, NE Wolins, G Serrero, EJ Blanchette-Mackie and C Londos
Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-2715, USA.

The adipose differentiation-related protein (ADRP) was first characterized as a mRNA induced early during adipocyte differentiation (Jiang, H. P., and G. Serrero. 1992. Proc. Natl. Acad. Sci. USA. 89:7856-7860). The present study demonstrates that ADRP mRNA is expressed in a variety of tissues and cultured cell lines. Immunocytochemical examination revealed that ADRP localizes to neutral lipid storage droplets in cultured murine 3T3-L1 adipocytes, murine MA- 10 Leydig cells, Chinese hamster ovary (CHO) fibroblasts, and human HepG2 hepatoma cells; the association of ADRP with lipid droplets was confirmed by subcellular fractionation of MA-10 Leydig cells. In addition to ADRP, steroidogenic cells and adipocytes express the perilipins, a family of lipid droplet-associated proteins that share a highly related sequence domain with ADRP. ADRP and perilipins co- localize on lipid droplets in MA-10 Leydig cells. While ADRP was found on small lipid droplets in 3T3-L1 preadipocytes and early differentiated adipocytes, it was absent in maturing adipocytes. In contrast, perilipins were absent early during differentiation, but were found on small and large lipid droplets at later stages. The transition in surface protein composition of adipocyte lipid droplets from ADRP to perilipins occurred 3 days after the initiation of differentiation when cells displayed co-localizatioin of both proteins on the same lipid droplets. The specific localization of adipose differentiation-related protein to lipid droplets in a wide variety of cells suggests that ADRP plays a role in management of neutral lipid stores.
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Adipose differentiation-related protein and regulators of lipid homeostasis identified by gene expression profiling in the murine db/db diabetic kidney
Am J Physiol Renal Physiol, May 1, 2004; 286(5): F913 - F921.
[Abstract] [Full Text] [PDF]

S. Urs, C. Smith, B. Campbell, A. M. Saxton, J. Taylor, B. Zhang, J. Snoddy, B. Jones Voy, and N. Moustaid-Moussa
Gene Expression Profiling in Human Preadipocytes and Adipocytes by Microarray Analysis
J. Nutr., April 1, 2004; 134(4): 762 - 770.
[Abstract] [Full Text] [PDF]

G. Larigauderie, C. Furman, M. Jaye, C. Lasselin, C. Copin, J.-C. Fruchart, G. Castro, and M. Rouis
Adipophilin Enhances Lipid Accumulation and Prevents Lipid Efflux From THP-1 Macrophages: Potential Role in Atherogenesis
Arterioscler Thromb Vasc Biol, March 1, 2004; 24(3): 504 - 510.
[Abstract] [Full Text]

A. Garcia, V. Subramanian, A. Sekowski, S. Bhattacharyya, M. W. Love, and D. L. Brasaemle
The Amino and Carboxyl Termini of Perilipin A Facilitate the Storage of Triacylglycerols
J. Biol. Chem., February 27, 2004; 279(9): 8409 - 8416.
[Abstract] [Full Text] [PDF]

A. G. Ostermeyer, L. T. Ramcharan, Y. Zeng, D. M. Lublin, and D. A. Brown
Role of the hydrophobic domain in targeting caveolin-1 to lipid droplets
J. Cell Biol., January 5, 2004; 164(1): 69 - 78.
[Abstract] [Full Text] [PDF]

I. Bildirici, C.-R. Roh, W. T. Schaiff, B. M. Lewkowski, D. M. Nelson, and Y. Sadovsky
The Lipid Droplet-Associated Protein Adipophilin Is Expressed in Human Trophoblasts and Is Regulated by Peroxisomal Proliferator-Activated Receptor-{gamma}/Retinoid X Receptor
J. Clin. Endocrinol. Metab., December 1, 2003; 88(12): 6056 - 6062.
[Abstract] [Full Text] [PDF]

X. Nan, J.-X. Cheng, and X. S. Xie
Vibrational imaging of lipid droplets in live fibroblast cells with coherent anti-Stokes Raman scattering microscopy
J. Lipid Res., November 1, 2003; 44(11): 2202 - 2208.
[Abstract] [Full Text] [PDF]

N. E. Wolins, J. R. Skinner, M. J. Schoenfish, A. Tzekov, K. G. Bensch, and P. E. Bickel
Adipocyte Protein S3-12 Coats Nascent Lipid Droplets
J. Biol. Chem., September 26, 2003; 278(39): 37713 - 37721.
[Abstract] [Full Text] [PDF]

M. Ohashi, N. Mizushima, Y. Kabeya, and T. Yoshimori
Localization of Mammalian NAD(P)H Steroid Dehydrogenase-like Protein on Lipid Droplets
J. Biol. Chem., September 19, 2003; 278(38): 36819 - 36829.
[Abstract] [Full Text] [PDF]

O. Gavrilova, M. Haluzik, K. Matsusue, J. J. Cutson, L. Johnson, K. R. Dietz, C. J. Nicol, C. Vinson, F. J. Gonzalez, and M. L. Reitman
Liver Peroxisome Proliferator-activated Receptor {gamma} Contributes to Hepatic Steatosis, Triglyceride Clearance, and Regulation of Body Fat Mass
J. Biol. Chem., September 5, 2003; 278(36): 34268 - 34276.
[Abstract] [Full Text] [PDF]

T. Hayashi and T.-P. Su
{sigma}-1 Receptors ({sigma}1 Binding Sites) Form Raft-Like Microdomains and Target Lipid Droplets on the Endoplasmic Reticulum: Roles in Endoplasmic Reticulum Lipid Compartmentalization and Export
J. Pharmacol. Exp. Ther., August 1, 2003; 306(2): 718 - 725.
[Abstract] [Full Text] [PDF]

T. Hayashi and T.-P. Su
Intracellular Dynamics of {sigma}-1 Receptors ({sigma}1 Binding Sites) in NG108-15 Cells
J. Pharmacol. Exp. Ther., August 1, 2003; 306(2): 726 - 733.
[Abstract] [Full Text] [PDF]

C. Sztalryd, G. Xu, H. Dorward, J. T. Tansey, J. A. Contreras, A. R. Kimmel, and C. Londos
Perilipin A is essential for the translocation of hormone-sensitive lipase during lipolytic activation
J. Cell Biol., June 23, 2003; 161(6): 1093 - 1103.
[Abstract] [Full Text] [PDF]

D. DiDonato and D. L. Brasaemle
Fixation Methods for the Study of Lipid Droplets by Immunofluorescence Microscopy
J. Histochem. Cytochem., June 1, 2003; 51(6): 773 - 780.
[Abstract] [Full Text] [PDF]

S. Rome, K. Clement, R. Rabasa-Lhoret, E. Loizon, C. Poitou, G. S. Barsh, J.-P. Riou, M. Laville, and H. Vidal
Microarray Profiling of Human Skeletal Muscle Reveals That Insulin Regulates ~800 Genes during a Hyperinsulinemic Clamp
J. Biol. Chem., May 9, 2003; 278(20): 18063 - 18068.
[Abstract] [Full Text] [PDF]

P. Targett-Adams, D. Chambers, S. Gledhill, R. G. Hope, J. F. Coy, A. Girod, and J. McLauchlan
Live Cell Analysis and Targeting of the Lipid Droplet-binding Adipocyte Differentiation-related Protein
J. Biol. Chem., April 25, 2003; 278(18): 15998 - 16007.
[Abstract] [Full Text] [PDF]

J. L. McManaman, W. Zabaronick, J. Schaack, and D. J. Orlicky
Lipid droplet targeting domains of adipophilin
J. Lipid Res., April 1, 2003; 44(4): 668 - 673.
[Abstract] [Full Text] [PDF]

J. T. Tansey, A. M. Huml, R. Vogt, K. E. Davis, J. M. Jones, K. A. Fraser, D. L. Brasaemle, A. R. Kimmel, and C. Londos
Functional Studies on Native and Mutated Forms of Perilipins. A ROLE IN PROTEIN KINASE A-MEDIATED LIPOLYSIS OF TRIACYLGLYCEROLS IN CHINESE HAMSTER OVARY CELLS
J. Biol. Chem., February 28, 2003; 278(10): 8401 - 8406.
[Abstract] [Full Text] [PDF]

M. Imamura, T. Inoguchi, S. Ikuyama, S. Taniguchi, K. Kobayashi, N. Nakashima, and H. Nawata
ADRP stimulates lipid accumulation and lipid droplet formation in murine fibroblasts
Am J Physiol Endocrinol Metab, October 1, 2002; 283(4): E775 - E783.
[Abstract] [Full Text] [PDF]

C. J. Schultz, E. Torres, C. Londos, and J. S. Torday
Role of adipocyte differentiation-related protein in surfactant phospholipid synthesis by type II cells
Am J Physiol Lung Cell Mol Physiol, August 1, 2002; 283(2): L288 - L296.
[Abstract] [Full Text] [PDF]

S. C. Souza, K. V. Muliro, L. Liscum, P. Lien, M. T. Yamamoto, J. E. Schaffer, G. E. Dallal, X. Wang, F. B. Kraemer, M. Obin, et al.
Modulation of Hormone-sensitive Lipase and Protein Kinase A-mediated Lipolysis by Perilipin A in an Adenoviral Reconstituted System
J. Biol. Chem., March 1, 2002; 277(10): 8267 - 8272.
[Abstract] [Full Text] [PDF]

N. B. Cole, D. D. Murphy, T. Grider, S. Rueter, D. Brasaemle, and R. L. Nussbaum
Lipid Droplet Binding and Oligomerization Properties of the Parkinson's Disease Protein alpha -Synuclein
J. Biol. Chem., February 15, 2002; 277(8): 6344 - 6352.
[Abstract] [Full Text] [PDF]

R. G. Hope, D. J. Murphy, and J. McLauchlan
The Domains Required to Direct Core Proteins of Hepatitis C Virus and GB Virus-B to Lipid Droplets Share Common Features with Plant Oleosin Proteins
J. Biol. Chem., February 1, 2002; 277(6): 4261 - 4270.
[Abstract] [Full Text] [PDF]

A. J. Charron and L. D. Sibley
Host cells: mobilizable lipid resources for the intracellular parasite Toxoplasma gondii
J. Cell Sci., January 8, 2002; 115(15): 3049 - 3059.
[Abstract] [Full Text] [PDF]

S. Cases, S. J. Stone, P. Zhou, E. Yen, B. Tow, K. D. Lardizabal, T. Voelker, and R. V. Farese Jr.
Cloning of DGAT2, a Second Mammalian Diacylglycerol Acyltransferase, and Related Family Members
J. Biol. Chem., October 12, 2001; 276(42): 38870 - 38876.
[Abstract] [Full Text] [PDF]

J. T. Tansey, C. Sztalryd, J. Gruia-Gray, D. L. Roush, J. V. Zee, O. Gavrilova, M. L. Reitman, C.-X. Deng, C. Li, A. R. Kimmel, et al.
Perilipin ablation results in a lean mouse with aberrant adipocyte lipolysis, enhanced leptin production, and resistance to diet-induced obesity
PNAS, May 22, 2001; 98(11): 6494 - 6499.
[Abstract] [Full Text] [PDF]

ARTICLES

Adipose differentiation-related protein is an ubiquitously expressed lipid storage droplet-associated protein