HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Parks, J. S. Articles by Gebre, A. K. Search for Related Content PubMed PubMed Citation Articles by Parks, J. S. Articles by Gebre, A. K. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 38, 266-275, Copyright © 1997 by Lipid Research, Inc.

ARTICLES

Long-chain polyunsaturated fatty acids in the sn-2 position of phosphatidylcholine decrease the stability of recombinant high density lipoprotein apolipoprotein A-I and the activation energy of the lecithin:cholesterol acyltransferase reaction

JS Parks and AK Gebre
Department of Comparative Medicine, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157-6279, USA.

The lecithin:cholesterol acyltransferase (LCAT) kinetics and activation energy and the stability of apolipoprotein A-I (apoA-I) were investigated using recombinant HDL (rHDL) containing phosphatidylcholine (PC), [3H]cholesterol, and apo A-I. The PC component of the rHDL contained sn-1 16:0 and sn-2 18:1 (POPC), 20:4 (PAPC), 20:5 n-3 (PEPC), or 22:6 n-3 (PDPC) or 10% of the respective PC species and 90% sn-1 18:1, sn-2 16:0 PC ether (OPPC ether). The appVmax of the rHDL containing 100% PC varied 10-fold and was ordered POPC > PEPC > PAPC > PDPC, whereas the appKm values varied 19-43 microns PC. The ether-containing rHDL had appVmax values 17-40% of their respective 100% PC rHDL, but maintained the same rank order. The activation energy of LCAT was lower for rHDL containing long chain polyunsaturated fatty acids (PUFA) compared to rHDL containing 100% POPC or 10% PC/90% OPPC ether. The concentration of guanidine HCl (D1/2) required to denature one-half of the apoA-I on rHDL containing long chain PUFA was reduced (1.2-16 M) compared to those containing 100% POPC or 10% PC/90% OPPC (2.2-2.4 M) and there was a strong correlation (r = 0.71) between LCAT activation energy and the stability of apoA-I (i.e., D1/2). We conclude that long chain PUFA in the sn-2 position of PC decreases the catalytic efficiency of LCAT, the activation energy of the LCAT reaction and the stability of apoA-I on the rHDL particles. The strong association between rHDL apoA-I stability and LCAT activation energy suggests that the temperature-dependent step of the LCAT reaction may be sensitive to the strength of the interaction of apoA-I with rHDL PC.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. Y. Lee, A. Lesimple, M. Denis, J. Vincent, A. Larsen, O. Mamer, L. Krimbou, J. Genest, and M. Marcil Increased sphingomyelin content impairs HDL biogenesis and maturation in human Niemann-Pick disease type B J. Lipid Res., March 1, 2006; 47(3): 622 - 632. [Abstract] [Full Text] [PDF]

				L. Krimbou, H. Hajj Hassan, S. Blain, S. Rashid, M. Denis, M. Marcil, and J. Genest Biogenesis and speciation of nascent apoA-I-containing particles in various cell lines J. Lipid Res., August 1, 2005; 46(8): 1668 - 1677. [Abstract] [Full Text] [PDF]

				Y. Zhao, A. K. Gebre, and J. S. Parks Amino acids 149 and 294 of human lecithin:cholesterol acyltransferase affect fatty acyl specificity J. Lipid Res., December 1, 2004; 45(12): 2310 - 2316. [Abstract] [Full Text] [PDF]

				J. W. Furbee Jr., O. Francone, and J. S. Parks In vivo contribution of LCAT to apolipoprotein B lipoprotein cholesteryl esters in LDL receptor and apolipoprotein E knockout mice J. Lipid Res., March 1, 2002; 43(3): 428 - 437. [Abstract] [Full Text] [PDF]

				N. D. Vaziri, K. Liang, and J. S. Parks Acquired lecithin-cholesterol acyltransferase deficiency in nephrotic syndrome Am J Physiol Renal Physiol, May 1, 2001; 280(5): F823 - F828. [Abstract] [Full Text] [PDF]

				M. G. Sorci-Thomas, M. Thomas, L. Curtiss, and M. Landrum Single Repeat Deletion in ApoA-I Blocks Cholesterol Esterification and Results in Rapid Catabolism of Delta 6 and Wild-type ApoA-I in Transgenic Mice J. Biol. Chem., April 14, 2000; 275(16): 12156 - 12163. [Abstract] [Full Text] [PDF]

				J. S. Parks, K. W. Huggins, A. K. Gebre, and E. R. Burleson Phosphatidylcholine fluidity and structure affect lecithin:cholesterol acyltransferase activity J. Lipid Res., April 1, 2000; 41(4): 546 - 553. [Abstract] [Full Text]

				J. W. Chisholm, A. K. Gebre, and J. S. Parks Characterization of C-terminal histidine-tagged human recombinant lecithin:cholesterol acyltransferase J. Lipid Res., August 1, 1999; 40(8): 1512 - 1519. [Abstract] [Full Text]

				K. W. Huggins, L. K. Curtiss, A. K. Gebre, and J. S. Parks Effect of long chain polyunsaturated fatty acids in the sn-2 position of phosphatidylcholine on the interaction with recombinant high density lipoprotein apolipoprotein A-I J. Lipid Res., December 1, 1998; 39(12): 2423 - 2431. [Abstract] [Full Text]

				C. T. DeMaria, Y. Sun, L. Long, B. J. Wagner, and G. Brewer Structural Determinants in AUF1 Required for High Affinity Binding to A + U-rich Elements J. Biol. Chem., October 31, 1997; 272(44): 27635 - 27643. [Abstract] [Full Text] [PDF]