HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by van Antwerpen, R. Articles by Gilkey, J. C. Search for Related Content PubMed PubMed Citation Articles by van Antwerpen, R. Articles by Gilkey, J. C. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 38, 659-669, Copyright © 1997 by Lipid Research, Inc.

ARTICLES

Cryo-electron microscopy of low density lipoprotein and reconstituted discoidal high density lipoprotein: imaging of the apolipoprotein moiety

R van Antwerpen, GC Chen, CR Pullinger, JP Kane, M LaBelle, RM Krauss, C Luna- Chavez, TM Forte and JC Gilkey
Department of Biochemistry, University of Arizona, Tucson 85721, USA.

Cryo-electron microscopy was used to analyze the structure of low density lipoprotein from normolipidemic subjects (N-LDL), phospholipid- depleted N-LDL (PD-LDL), small dense LDL from hypertriglyceridemic subjects (SD-LDL), and reconstituted discoidal high density lipoproteins (rHDL). In different projections of N-LDL, a high density component of the particle was visible as two parallel bands or as a single ring. Projections of PD-LDL were very similar to those of N-LDL, indicating that the contribution of phospholipid headgroups to the observed high density structure is minor. In preparations of SD-LDL, projections with two high density bands or a single high density ring were rare. Instead, triangular and diamond-shaped projections were recognized. In different projections of discoidal rHDL, a high density component was visible as a single band or as a single ring. The present results indicate that cryo-electron microscopy reveals the distribution of apolipoproteins within lipoprotein particles. Thus, apolipoprotein B- 100 (apoB) in N-LDL appears to be organized as a double ring around the particle, while apoB in SD-LDL is indicated to have a different conformation. Cryo-electron micrographs of rHDL are consistent with the presence of apolipoprotein A-I on the periphery of the lipoprotein disc.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				T. Teerlink, P. G. Scheffer, S. J. L. Bakker, and R. J. Heine Combined data from LDL composition and size measurement are compatible with a discoid particle shape J. Lipid Res., May 1, 2004; 45(5): 954 - 966. [Abstract] [Full Text] [PDF]

				J. P. Segrest, M. K. Jones, H. De Loof, and N. Dashti Structure of apolipoprotein B-100 in low density lipoproteins J. Lipid Res., September 1, 2001; 42(9): 1346 - 1367. [Abstract] [Full Text] [PDF]

				R. van Antwerpen, M. La Belle, E. Navratilova, and R. M. Krauss Structural heterogeneity of apoB-containing serum lipoproteins visualized using cryo-electron microscopy J. Lipid Res., October 1, 1999; 40(10): 1827 - 1836. [Abstract] [Full Text]

				E. V. Orlova, M. B. Sherman, W. Chiu, H. Mowri, L. C. Smith, and A. M. Gotto Jr. Three-dimensional structure of low density lipoproteins by electron cryomicroscopy PNAS, July 20, 1999; 96(15): 8420 - 8425. [Abstract] [Full Text] [PDF]