| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Lipid Research, Vol 38, 723-733, Copyright © 1997 by Lipid Research, Inc.
HA Jurevics, FZ Kidwai and P Morell
Female rats at various stages of pregnancy were injected intraperitoneally
with [3H]water; 4 h later, they were killed, the uterus was removed, and
the fetuses were dissected. Lipids were isolated and fractionated by HPLC
and the total amount of cholesterol in each organ, as well as radioactivity
incorporated into cholesterol and cholesterol precursors, were determined.
From the data for cholesterol content at each age we calculated the rate of
accumulation of cholesterol during fetal development. As incorporation of
label from [3H]water takes place with a stoichiometry defined by a known
biosynthetic pathway, we were also able to determine the fraction of
cholesterol accumulating in each organ that had been newly synthesized. For
the fetus as a whole, more than 93% of the cholesterol accumulating during
development was newly synthesized. As the specific radioactivity of
cholesterol in the maternal circulation was negligible (because synthesis
of cholesterol by maternal liver was suppressed by inclusion of cholesterol
in the diet), we conclude that the fetus synthesizes nearly all of its own
cholesterol; neither the maternal circulation nor the placenta/yolk sac
contribute significant amounts of cholesterol to the fetus. We were also
able to quantitate trafficking of cholesterol between fetal organs. Fetal
brain is responsible for the synthesis of all of its own cholesterol. In
contrast, fetal liver exports cholesterol into the fetal circulation and
supplies about half of the cholesterol for development of heart, lung, and
kidney.
ARTICLES
Sources of cholesterol during development of the rat fetus and fetal organs
Neuroscience Center, University of North Carolina, Chapel Hill 27559- 7250, USA.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Guizzetti, J. Chen, J. F. Oram, R. Tsuji, K. Dao, T. Moller, and L. G. Costa Ethanol Induces Cholesterol Efflux and Up-regulates ATP-binding Cassette Cholesterol Transporters in Fetal Astrocytes J. Biol. Chem., June 29, 2007; 282(26): 18740 - 18749. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Guizzetti and L. Costa Cholesterol homeostasis in the developing brain: a possible new target for ethanol Human and Experimental Toxicology, April 1, 2007; 26(4): 355 - 360. [Abstract] [PDF]
|
|
|
|
 |
|
 G. S. Tint, H. Yu, Q. Shang, G. Xu, and S. B. Patel The use of the Dhcr7 knockout mouse to accurately determine the origin of fetal sterols J. Lipid Res., July 1, 2006; 47(7): 1535 - 1541. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. S. Correa-Cerro, C. A. Wassif, L. Kratz, G. F. Miller, J. P. Munasinghe, A. Grinberg, S. J. Fliesler, and F. D. Porter Development and characterization of a hypomorphic Smith-Lemli-Opitz syndrome mouse model and efficacy of simvastatin therapy Hum. Mol. Genet., March 15, 2006; 15(6): 839 - 851. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yoshida and Y. Wada Transfer of maternal cholesterol to embryo and fetus in pregnant mice J. Lipid Res., October 1, 2005; 46(10): 2168 - 2174. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zhang, R. M. Lewis, C. Wang, N. Hales, and C. D. Byrne Maternal dietary iron restriction modulates hepatic lipid metabolism in the fetuses Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2005; 288(1): R104 - R111. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Keller, M. Small, and S. J. Fliesler Enzyme blockade: a nonradioactive method to determine the absolute rate of cholesterol synthesis in the brain J. Lipid Res., October 1, 2004; 45(10): 1952 - 1957. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Beigneux, C. Kosinski, B. Gavino, J. D. Horton, W. C. Skarnes, and S. G. Young ATP-Citrate Lyase Deficiency in the Mouse J. Biol. Chem., March 5, 2004; 279(10): 9557 - 9564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Schmid, W. S. Davidson, L. Myatt, and L. A. Woollett Transport of cholesterol across a BeWo cell monolayer: implications for net transport of sterol from maternal to fetal circulation J. Lipid Res., October 1, 2003; 44(10): 1909 - 1918. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Xie, E. G. Lund, S. D. Turley, D. W. Russell, and J. M. Dietschy Quantitation of two pathways for cholesterol excretion from the brain in normal mice and mice with neurodegeneration J. Lipid Res., September 1, 2003; 44(9): 1780 - 1789. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Rigotti, H. E. Miettinen, and M. Krieger The Role of the High-Density Lipoprotein Receptor SR-BI in the Lipid Metabolism of Endocrine and Other Tissues Endocr. Rev., June 1, 2003; 24(3): 357 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Soria, C. Bocos, and E. Herrera Opposite metabolic response to fenofibrate treatment in pregnant and virgin rats J. Lipid Res., January 1, 2002; 43(1): 74 - 81. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Wassif, P. Zhu, L. Kratz, P. A. Krakowiak, K. P. Battaile, F. F. Weight, A. Grinberg, R. D. Steiner, N. A. Nwokoro, R. I. Kelley, et al. Biochemical, phenotypic and neurophysiological characterization of a genetic mouse model of RSH/Smith-Lemli-Opitz syndrome Hum. Mol. Genet., March 1, 2001; 10(6): 555 - 564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. I. Posse de Chaves, D. E. Vance, R. B. Campenot, R. S. Kiss, and J. E. Vance Uptake of Lipoproteins for Axonal Growth of Sympathetic Neurons J. Biol. Chem., June 23, 2000; 275(26): 19883 - 19890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. McConihay, A. M. Honkomp, N. A. Granholm, and L. A. Woollett Maternal high density lipoproteins affect fetal mass and extra-embryonic fetal tissue sterol metabolism in the mouse J. Lipid Res., March 1, 2000; 41(3): 424 - 432. [Abstract] [Full Text]
|
|
|
|
|
|
R.-i. Tozawa, S. Ishibashi, J.-i. Osuga, H. Yagyu, T. Oka, Z. Chen, K. Ohashi, S. Perrey, F. Shionoiri, N. Yahagi, et al. Embryonic Lethality and Defective Neural Tube Closure in Mice Lacking Squalene Synthase J. Biol. Chem., October 22, 1999; 274(43): 30843 - 30848. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Xie, S. D. Turley, and J. M. Dietschy Cholesterol accumulation in tissues of the Niemann-Pick type C mouse is determined by the rate of lipoprotein-cholesterol uptake through the coated-pit pathway in each organ PNAS, October 12, 1999; 96(21): 11992 - 11997. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Xie, S. D. Turley, P. G. Pentchev, and J. M. Dietschy Cholesterol balance and metabolism in mice with loss of function of Niemann-Pick C protein Am J Physiol Endocrinol Metab, February 1, 1999; 276(2): E336 - E344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Hatzopoulos, A. Rigotti, R. D. Rosenberg, and M. Krieger Temporal and spatial pattern of expression of the HDL receptor SR-BI during murine embryogenesis J. Lipid Res., March 1, 1998; 39(3): 495 - 508. [Abstract] [Full Text]
|
|
|
|
|
|
K. L. Wyne and L. A. Woollett Transport of maternal LDL and HDL to the fetal membranes and placenta of the Golden Syrian hamster is mediated by receptor-dependent and receptor-independent processes J. Lipid Res., March 1, 1998; 39(3): 518 - 530. [Abstract] [Full Text]
|
|
|
|
|
|
L. P. Henderson, L. Lin, A. Prasad, C. A. Paul, T. Y. Chang, and R. A. Maue Embryonic Striatal Neurons from Niemann-Pick Type C Mice Exhibit Defects in Cholesterol Metabolism and Neurotrophin Responsiveness J. Biol. Chem., June 23, 2000; 275(26): 20179 - 20187. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
