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Journal of Lipid Research, Vol 38, 734-744, Copyright © 1997 by Lipid Research, Inc.
Modification of the cholesterol efflux properties of human serum by enrichment with phospholipid
B Jian, M de la Llera-Moya, L Royer, G Rothblat, O Francone and JB Swaney
Department of Biochemistry, Allegheny University of the Health Sciences, Philadelphia, PA 19129, USA.
To investigate the importance of phospholipid in promoting cholesterol
efflux from cells, phospholipid multilamellar vesicles were incubated with
normal human serum and the efflux ability of these lipid-modified sera was
tested. When incubated under appropriate conditions, both
dimyristoylphosphatidylcholine (DMPC) and bovine brain sphingomyelin (BBSM)
were shown to combine with components of human serum to form new
protein:lipid complexes and to markedly enhance the ability of serum to
promote efflux of cholesterol from Fu5AH cells. In particular, the high
density lipoprotein (HDL) particles were altered in their composition and
electrophoretic properties and the alpha-migrating species, which were
reactive with antibodies to apo-A-I, were converted to larger, pre-
beta-migrating particles, similar in electrophoretic properties to pre
beta(2)-HDL. DMPC, but not BBSM, also generated particles with mobility
similar to pre beta(2)-HDL; These species were demonstrably different from
the discoidal complexes formed by reaction of DMPC with purified apoA-I.
However, no change in cholesterol efflux potential was observed when serum
was mixed with phospholipids that failed to interact or when cells were
incubated with phospholipid multilamellar vesicles alone. To further
identify the components of serum that become altered in their efflux
potential after reaction with phospholipid, isolated lipoprotein fractions
were incubated with DMPC or BBSM and it was found that only interaction
with HDL caused enhancement of cholesterol efflux. In summary, cholesterol
removal from the Fu5AH cells by serum can be promoted by adding
phospholipid under conditions where new HDL-like complexes can be formed
between the phospholipid and serum components, most notably apolipoprotein
A-I.

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Copyright © 1997 by the American Society for Biochemistry and Molecular Biology.
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