Journal of Lipid Research, Vol 38, 795-804, Copyright © 1997 by Lipid Research, Inc.

ARTICLES

Variation in adrenergic regulation of lipolysis between omental and subcutaneous adipocytes from obese and non-obese men

J Hoffstedt, P Arner, G Hellers and F Lonnqvist
Department of Medicine and Surgery, Huddinge University Hospital, Sweden.

Regional variations in adipocyte lipolysis between subcutaneous and visceral fat may be important for obesity complications. In the present study, we compared adrenergic regulation of lipolysis in omental and subcutaneous adipocytes from obese (n = 15) and non-obese (n = 14) male subjects. Waist-to-hip ratio, blood pressure, plasma insulin, and plasma triglycerides were increased in obesity. No regional differences in adrenoceptor lipolytic function were observed in non-obese subjects with the exception of a slight increase in noradrenaline sensitivity in omental adipocytes (P < 0.05), because of increased beta(1)- adrenoceptor sensitivity (P < 0.05). In the obese subjects, the rate of noradrenaline-induced glycerol release was 2-fold higher (P < 0.005) and the noradrenaline sensitivity was 3-fold higher (P < 0.05) in omental versus subcutaneous adipocytes. These findings were mainly due to a 50-fold increase in omental beta(3)-adrenoceptor sensitivity (P < 0.002) and to a smaller 6-fold increase in omental beta(1)-adrenoceptor sensitivity (P < 0.02), accompanied by increased beta(3)- as well as beta(1)-adrenoceptor lipolytic rates at approximately 50% receptor subtype occupancy by the agonist (P < 0.05). In conclusion, minor regional differences in adipocyte lipolytic response to catecholamines are present in non-obese males. In contrast, catecholamine-induced lipolysis is markedly increased in omental as compared to subcutaneous adipocytes in obese males, mainly due to an increase in beta(3)- adrenoceptor function of visceral fat cells, in combination with a smaller increase in beta(1)-adrenoceptor function.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				P. U. Freda, W. Shen, S. B. Heymsfield, C. M. Reyes-Vidal, E. B. Geer, J. N. Bruce, and D. Gallagher Lower Visceral and Subcutaneous but Higher Intermuscular Adipose Tissue Depots in Patients with Growth Hormone and Insulin-Like Growth Factor I Excess Due to Acromegaly J. Clin. Endocrinol. Metab., June 1, 2008; 93(6): 2334 - 2343. [Abstract] [Full Text] [PDF]

				R. MacLaren, W. Cui, S. Simard, and K. Cianflone Influence of obesity and insulin sensitivity on insulin signaling genes in human omental and subcutaneous adipose tissue J. Lipid Res., February 1, 2008; 49(2): 308 - 323. [Abstract] [Full Text] [PDF]

				K. Kos, A. L. Harte, S. James, D. R. Snead, J. P. O'Hare, P. G. McTernan, and S. Kumar Secretion of neuropeptide Y in human adipose tissue and its role in maintenance of adipose tissue mass Am J Physiol Endocrinol Metab, November 1, 2007; 293(5): E1335 - E1340. [Abstract] [Full Text] [PDF]

				A. Tchernof, C. Belanger, A.-S. Morisset, C. Richard, J. Mailloux, P. Laberge, and P. Dupont Regional differences in adipose tissue metabolism in women: minor effect of obesity and body fat distribution. Diabetes, May 1, 2006; 55(5): 1353 - 1360. [Abstract] [Full Text] [PDF]

				S. W. Coppack, D. L. Chinkes, J. M. Miles, B. W. Patterson, and S. Klein A Multicompartmental Model of In Vivo Adipose Tissue Glycerol Kinetics and Capillary Permeability in Lean and Obese Humans Diabetes, July 1, 2005; 54(7): 1934 - 1941. [Abstract] [Full Text] [PDF]

				B. Mittendorfer Sexual Dimorphism in Human Lipid Metabolism J. Nutr., April 1, 2005; 135(4): 681 - 686. [Abstract] [Full Text] [PDF]

				B. Mittendorfer, D. A. Fields, and S. Klein Excess body fat in men decreases plasma fatty acid availability and oxidation during endurance exercise Am J Physiol Endocrinol Metab, March 1, 2004; 286(3): E354 - E362. [Abstract] [Full Text]

				Y. Li, H. Bujo, K. Takahashi, M. Shibasaki, Y. Zhu, Y. Yoshida, Y. Otsuka, N. Hashimoto, and Y. Saito Visceral Fat: Higher Responsiveness of Fat Mass and Gene Expression to Calorie Restriction than Subcutaneous Fat Experimental Biology and Medicine, November 1, 2003; 228(10): 1118 - 1123. [Abstract] [Full Text] [PDF]

				Y. Miyazaki, L. Glass, C. Triplitt, E. Wajcberg, L. J. Mandarino, and R. A. DeFronzo Abdominal fat distribution and peripheral and hepatic insulin resistance in type 2 diabetes mellitus Am J Physiol Endocrinol Metab, December 1, 2002; 283(6): E1135 - E1143. [Abstract] [Full Text] [PDF]

				C. Punyadeera, M-T. van der Merwe, N. J. Crowther, M. Toman, G. P. Schlaphoff, and I. P. Gray Ethnic differences in lipid metabolism in two groups of obese South African women J. Lipid Res., May 1, 2001; 42(5): 760 - 767. [Abstract] [Full Text]

				L. H. Enevoldsen, B. Stallknecht, J. D. Fluckey, and H. Galbo Effect of exercise training on in vivo lipolysis in intra-abdominal adipose tissue in rats Am J Physiol Endocrinol Metab, September 1, 2000; 279(3): E585 - E592. [Abstract] [Full Text] [PDF]

				D. E. Kelley, F. L. Thaete, F. Troost, T. Huwe, and B. H. Goodpaster Subdivisions of subcutaneous abdominal adipose tissue and insulin resistance Am J Physiol Endocrinol Metab, May 1, 2000; 278(5): E941 - E948. [Abstract] [Full Text] [PDF]

				J. Saleh, N. Christou, and K. Cianflone Regional specificity of ASP binding in human adipose tissue Am J Physiol Endocrinol Metab, May 1, 1999; 276(5): E815 - E821. [Abstract] [Full Text] [PDF]

				P. Mauriège, M. Brochu, D. Prud’homme, A. Tremblay, A. Nadeau, S. Lemieux, and J. P. Després Is Visceral Adiposity a Significant Correlate of Subcutaneous Adipose Cell Lipolysis in Men? J. Clin. Endocrinol. Metab., February 1, 1999; 84(2): 736 - 742. [Abstract] [Full Text]

				J. R. Sowers Obesity and cardiovascular disease Clin. Chem., August 1, 1998; 44(8): 1821 - 1825. [Abstract] [Full Text] [PDF]

				S. Reynisdottir, M. Dauzats, A. Thorne, and D. Langin Comparison of Hormone-Sensitive Lipase Activity in Visceral and Subcutaneous Human Adipose Tissue J. Clin. Endocrinol. Metab., December 1, 1997; 82(12): 4162 - 4166. [Abstract] [Full Text] [PDF]