Journal of Lipid Research, Vol 38, 1048-1052, Copyright © 1997 by Lipid Research, Inc.

ARTICLES

A new method for the rapid measurement of cholesterol crystallization in model biles using a spectrophotometric microplate reader

GJ Somjen, Y Ringel, FM Konikoff, R Rosenberg and T Gilat
Department of Gastroenterology, Souraski Medical Center, Ichilov Hospital, Tel Aviv, Israel.

Measurements of the cholesterol crystal observation time, and particularly the crystal growth rate in model biles, are important in biliary pathophysiology. The aim of this study was to develop a semi- automated method permitting multiple, simultaneous, and precise measurements of the crystal growth rate in model biles. Incubated model biles were mixed with a high concentration of NaTDC to solubilize non- crystalline turbidity and spectrophotometric measurements were performed. In parallel, samples were observed by light microscopy. The absorbance correlated linearly with the crystal mass and permitted quantitation of the crystal growth rate. Polarized light microscopy was more sensitive than spectrophotometry for determining the initial crystal observation time, while spectrophotometry was more precise and quantitative for measuring the crystal growth rate.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				P. Portincasa, N. G. Venneman, A. Moschetta, A. van den Berg, G. Palasciano, G. P. vanBerge-Henegouwen, and K. J. van Erpecum Quantitation of cholesterol crystallization from supersaturated model bile J. Lipid Res., April 1, 2002; 43(4): 604 - 610. [Abstract] [Full Text] [PDF]

				A. Moschetta, G. P. vanBerge-Henegouwen, P. Portincasa, G. Palasciano, and K. J. van Erpecum Cholesterol crystallization in model biles: effects of bile salt and phospholipid species composition J. Lipid Res., August 1, 2001; 42(8): 1273 - 1281. [Abstract] [Full Text] [PDF]