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Journal of Lipid Research, Vol 38, 1254-1260, Copyright © 1997 by Lipid Research, Inc.
AB Reiss, KO Martin, DE Rojer, S Iyer, EA Grossi, AC Galloway and NB Javitt
Human endothelium obtained from both the aorta and the pulmonary artery has
been evaluated for the presence of the messenger RNA coding for the
expression of sterol 27-hydroxylase. Unique oligomers were designed to
detect the mRNA by reverse transcription followed by the polymerase chain
reaction. The amplified product was sequenced and was found to be identical
to the published sequence for nucleotides 491 to 802 of the human sterol
27-hydroxylase cDNA. Northern blot analysis confirmed the presence of
27-hydroxylase mRNA in pulmonary artery and aortic endothelium. As part of
these studies, enzymatic activity was assayed in cultured arterial
endothelium using cholesterol as a substrate and isotope ratio gas-liquid
chromatography-mass spectrometry to identify the metabolites,
27-hydroxycholesterol and 3 beta-hydroxy-5- cholestenoic acid, in the
medium. Localization of sterol 27-hydroxylase to vascular endothelium
indicates intracellular production of the biologically active metabolite
27-hydroxycholesterol.
ARTICLES
Sterol 27-hydroxylase: expression in human arterial endothelium
Division of Hepatic Diseases, New York University Medical Center, NY 10016, USA.
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