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Journal of Lipid Research, Vol 38, 1261-1266, Copyright © 1997 by Lipid Research, Inc.
DL Rainwater, PH Moore Jr, WR Shelledy, TD Dyer and SH Slifer
We describe a protocol for making a new type of gradient gel, the Composite
gradient gel, that was designed to resolve plasma lipoproteins using
nondenaturing gradient gel electrophoresis. The new gel format allows
analysis both of high density lipoproteins (HDLs) and low density
lipoproteins (LDLs) on the same gel. The gel gave highly repeatable (r2 =
0.999) size estimates. We compared lipoprotein phenotypes determined from
the new gradient gel with those obtained using specialized HDL and LDL
gradient gels. The comparisons indicated that the Composite gel gave
lipoprotein particle size estimates for HDLs and LDLs that were virtually
identical to those obtained, respectively, from the specialized HDL and LDL
gradient gels. We measured median diameters, which reflect the
distributions of absorbance, for LDLs and for HDLs and found that the
Composite gel gave lipoprotein size distributions that were virtually
identical to those measured using the specialized LDL and HDL gels.
Finally, comparison of fractional absorbance for six lipoprotein size
intervals obtained from the Composite and specialized gels revealed a close
correlation (r2 = 0.828). Thus, it appears that both LDL and HDL size
phenotypes may be evaluated simultaneously using a single gradient gel
format.
ARTICLES
Characterization of a composite gradient gel for the electrophoretic separation of lipoproteins
Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, TX 78245, USA.
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