HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Arnulphi, C. Articles by Fidelio, G. D. Search for Related Content PubMed PubMed Citation Articles by Arnulphi, C. Articles by Fidelio, G. D. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol 38, 1412-1420, Copyright © 1997 by Lipid Research, Inc.

ARTICLES

Ganglioside hydration study by 2H-NMR: dependence on temperature and water/lipid ratio

C Arnulphi, PR Levstein, ME Ramia, CA Martin and GD Fidelio
Facultad de Matematica, Astronomia y Fisica, Departamento de Quimica Biologica-CIQUIBIC, Ciudad Universitaria C.C., Cordoba, Argentina.

Dynamic properties of 2H2O in samples of ganglioside aggregates hydrated at water/lipid ratios ranging from 25:1 to 8000:1 mole/mole were studied by using deuterium nuclear magnetic resonance (2H-NMR). We present a physical model for the interpretation of the measured spin- spin relaxation times (T2). For all the concentrations studied the model provides evidence for the existence of at least two kinds of water environments: one in which the rotational correlation time is in the range of 10(-9) to 10(-8) s, and a second in which it lies between 10(-11) to 10(-10) s. A detailed study on the temperature dependence was performed for two of the concentrations, one corresponding to the hexagonal phase (100:1 mole/mole) and the other involving a micellar phase (200:1 mole/mole). In the 100:1 2H2O/ganglioside molar ratio sample, most of the water is tightly bound to long cylindrical structures. For the 200: 1 sample, there are on average approximately 30 water molecules tightly bound to the polar head group of each ganglioside molecule. The relative number and dynamics of molecules in this environment are essentially insensitive to temperature variations in the range 220-300K The rest of water molecules are also influenced by the aggregate, having a different mobility from that observed in the free liquid state.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Hirai, H. Iwase, T. Hayakawa, M. Koizumi, and H. Takahashi Determination of Asymmetric Structure of Ganglioside-DPPC Mixed Vesicle Using SANS, SAXS, and DLS Biophys. J., September 1, 2003; 85(3): 1600 - 1610. [Abstract] [Full Text] [PDF]