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Journal of Lipid Research, Vol 38, 1702-1706, Copyright © 1997 by Lipid Research, Inc.

ARTICLES

A two-dimensional thin-layer chromatography procedure for simultaneous separation of ceramide and diacylglycerol species

J Bodennec, G Brichon, O Koul, M El Babili and G Zwingelstein
Institut Michel Pacha, Universite Claude Bernard, Lyon I, La Seyne sur Mer, France.

We have developed a novel, simple, and rapid, two-dimensional thin- layer chromatography method to separate 1,2-, 1,3-diacylglycerols and ceramides containing alpha-hydroxy and normal fatty acids from other neutral lipids on one 10 x 10 cm precoated silica gel plate. The three solvent systems used in succession leave the phospholipids at the origin and separate neutral lipids of interest into component species. We have applied this method to incorporation of 9,10-[3H]myristic acid into lipids of gills from sea bass and obtained results that are similar and comparable to those obtained by described methods.
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