J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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The Journal of Lipid Research, Vol. 39, 218-227, January 1998
Copyright © 1998 by Lipid Research, Inc.

Original Article

Analysis of high density lipoprotein apolipoproteins by capillary zone and capillary SDS gel electrophoresis

Joseph Stocksa, M. Nazeem Nanjeea, and Norman E. Millera
a Department of Cardiovascular Biochemistry, St. Bartholomew's and the Royal London School of Medicine and Dentistry, Charterhouse Square, London, EC1M 6BQ, United Kingdom

Correspondence to: Norman E. Miller.

We describe new methods for analyzing the apolipoproteins (apo) of the high density lipoproteins (HDL) of several species by two modes of capillary electrophoresis: size separation using a molecular sieving buffer, and capillary zone electrophoresis (CZE) using neutral coated capillaries. By either mode HDL apos were resolved within 25 min. Results for apoA-I and apoA-II mass agreed with those by electroimmunoassay; intra-assay coefficients of variation were 1.8–4.2%. The migration times of human, rat, rabbit, and bovine apoA-I during CZE were proportional to their net charge/Mr ratios. This enabled human and rabbit apoA-I to be quantified simultaneously in transgenic rabbit HDLs. CZE also resolved human apoA-I isoforms, deamidated apoA-I, and pro-apoA-I.—Stocks, J., M. N. Nanjee, and N. E. Miller. Analysis of high density lipoprotein apolipoproteins by capillary zone and capillary SDS gel electrophoresis. J. Lipid Res. 1998. 39: 218–227.

Supplementary key words: apolipoprotein A-I, apolipoprotein A-II, sodium dodecyl sulfate, zone electrophoresis, bovine, immunoelectrophoresis, rabbit, rat, pro-apolipoprotein A-I


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