|
|
||||||||
Correspondence to:
L. M. Salati.
Expression of the glucose-6-phosphate dehydrogenase (G6PD) gene is inhibited by the addition of polyunsaturated fatty acids to the medium of primary hepatocytes in culture. To define the regulated step, we measured the abundance of G6PD mRNA both in the nucleus and in total RNA and measured the transcriptional activity of the G6PD gene. Insulin and glucose stimulated a 5- to 7-fold increase in G6PD mRNA in rat hepatocytes. This increase was attenuated by 60% due to the addition of arachidonic acid. These changes in mRNA accumulation occurred in the absence of changes in the rate of transcription. Amounts of precursor mRNA (pre-mRNA) for G6PD in the nucleus changed in parallel with the amount of mature mRNA. The decrease in G6PD pre-mRNA accumulation caused by arachidonic acid was also observed with other long chain polyunsaturated fatty acids but not with monounsaturated fatty acids. In addition, this decrease was not due to a generalized toxicity of the cells due to fatty acid oxidation.
These changes in G6PD expression in the primary hepatocytes are qualitatively and quantitatively similar to the changes observed in the intact animal due to dietary carbohydrate and polyunsaturated fat. Regulation of G6PD expression by a nuclear posttranscriptional mechanism represents a novel form of regulation by fatty acids.Stabile, L. P., S. A. Klautky, S. M. Minor, and L. M. Salati. Polyunsaturated fatty acids inhibit the expression of the glucose-6-phosphate dehydrogenase gene in primary rat hepatocytes by a nuclear posttranscriptional mechanism. J. Lipid Res. 1998. 39: 19511963.
Supplementary key words:
glucose-6-phosphate dehydrogenase, polyunsaturated fatty acids, rat hepatocytes, posttranscriptional regulation, nutritional regulation, precursor mRNA, RNA processing
Copyright © 1998 by Lipid Research, Inc.
Polyunsaturated fatty acids inhibit the expression of the glucose-6-phosphate dehydrogenase gene in primary rat hepatocytes by a nuclear posttranscriptional mechanism
L. P. Stabilea,
S. A. Klautkya,
S. M. Minora, and
L. M. Salatia
a Department of Biochemistry, West Virginia University, Health Sciences Center, Morgantown, WV 26506
![]()
CiteULike
Complore
Connotea
Del.icio.us
Digg
Reddit
Technorati What's this?
This article has been cited by other articles:
![]() |
S. Talukdar, S. Bhatnagar, S. Dridi, and F. B. Hillgartner Chenodeoxycholic acid suppresses the activation of acetyl-coenzyme A carboxylase-{alpha} gene transcription by the liver X receptor agonist T0-901317 J. Lipid Res., December 1, 2007; 48(12): 2647 - 2663. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Xu, K. Chakravarty, X. Kong, T. T. Tuy, I. J. Arinze, F. Bone, and D. Massillon Several Transcription Factors Are Recruited to the Glucose-6-Phosphatase Gene Promoter in Response to Palmitate in Rat Hepatocytes and H4IIE Cells J. Nutr., March 1, 2007; 137(3): 554 - 559. [Abstract] [Full Text] [PDF] |
||||
![]() |
W. Szeszel-Fedorowicz, I. Talukdar, B. N. Griffith, C. M. Walsh, and L. M. Salati An Exonic Splicing Silencer Is Involved in the Regulated Splicing of Glucose 6-Phosphate Dehydrogenase mRNA J. Biol. Chem., November 10, 2006; 281(45): 34146 - 34158. [Abstract] [Full Text] [PDF] |
||||
![]() |
I. Talukdar, W. Szeszel-Fedorowicz, and L. M. Salati Arachidonic Acid Inhibits the Insulin Induction of Glucose-6-phosphate Dehydrogenase via p38 MAP Kinase J. Biol. Chem., December 9, 2005; 280(49): 40660 - 40667. [Abstract] [Full Text] [PDF] |
||||
![]() |
L. M. Salati, W. Szeszel-Fedorowicz, H. Tao, M. A. Gibson, B. Amir-Ahmady, L. P. Stabile, and D. L. Hodge Nutritional Regulation of mRNA Processing J. Nutr., September 1, 2004; 134(9): 2437S - 2443S. [Abstract] [Full Text] [PDF] |
||||
![]() |
L. Siculella, F. Damiano, S. Sabetta, and G. V. Gnoni n-6 PUFAs downregulate expression of the tricarboxylate carrier in rat liver by transcriptional and posttranscriptional mechanisms J. Lipid Res., July 1, 2004; 45(7): 1333 - 1340. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. M. Giudetti, S. Sabetta, R. di Summa, M. Leo, F. Damiano, L. Siculella, and G. V. Gnoni Differential effects of coconut oil- and fish oil-enriched diets on tricarboxylate carrier in rat liver mitochondria J. Lipid Res., November 1, 2003; 44(11): 2135 - 2141. [Abstract] [Full Text] [PDF] |
||||
![]() |
L. Wang, A. R Folsom, Z.-J. Zheng, J. S Pankow, and J. H Eckfeldt Plasma fatty acid composition and incidence of diabetes in middle-aged adults: the Atherosclerosis Risk in Communities (ARIC) Study Am. J. Clinical Nutrition, July 1, 2003; 78(1): 91 - 98. [Abstract] [Full Text] [PDF] |
||||
![]() |
W. M. Frederiks, K. S. Bosch, J. S.S.G. De Jong, and C. J.F. Van Noorden Post-translational Regulation of Glucose-6-phosphate Dehydrogenase Activity in (Pre)neoplastic Lesions in Rat Liver J. Histochem. Cytochem., January 1, 2003; 51(1): 105 - 112. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. Tao, W. Szeszel-Fedorowicz, B. Amir-Ahmady, M. A. Gibson, L. P. Stabile, and L. M. Salati Inhibition of the Splicing of Glucose-6-phosphate Dehydrogenase Precursor mRNA by Polyunsaturated Fatty Acids J. Biol. Chem., August 16, 2002; 277(34): 31270 - 31278. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. K. Stoeckman and H. C. Towle The Role of SREBP-1c in Nutritional Regulation of Lipogenic Enzyme Gene Expression J. Biol. Chem., July 19, 2002; 277(30): 27029 - 27035. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. K. Mater, A. P. Thelen, D. A. Pan, and D. B. Jump Sterol Response Element-binding Protein 1c (SREBP1c) Is Involved in the Polyunsaturated Fatty Acid Suppression of Hepatic S14 Gene Transcription J. Biol. Chem., November 12, 1999; 274(46): 32725 - 32732. [Abstract] [Full Text] [PDF] |
||||
![]() |
J. Xu, M. Teran-Garcia, J. H. Y. Park, M. T. Nakamura, and S. D. Clarke Polyunsaturated Fatty Acids Suppress Hepatic Sterol Regulatory Element-binding Protein-1 Expression by Accelerating Transcript Decay J. Biol. Chem., March 23, 2001; 276(13): 9800 - 9807. [Abstract] [Full Text] [PDF] |
||||
![]() |
B. Amir-Ahmady and L. M. Salati Regulation of the Processing of Glucose-6-phosphate Dehydrogenase mRNA by Nutritional Status J. Biol. Chem., March 23, 2001; 276(13): 10514 - 10523. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |