HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gillotte, K. L. Articles by Phillips, M. C. Search for Related Content PubMed PubMed Citation Articles by Gillotte, K. L. Articles by Phillips, M. C. Social Bookmarking                      What's this?

The Journal of Lipid Research, Vol. 39, 2065-2075, October 1998
Copyright © 1998 by Lipid Research, Inc.

Dietary modification of high density lipoprotein phospholipid and influence on cellular cholesterol efflux

Correspondence to: Michael C. Phillips.

African green monkeys fed fat-specific diets served as a model to investigate the effect of phospholipid acyl chain modification on high density lipoprotein (HDL)-mediated cellular cholesterol efflux. Diets enriched in saturated, monounsaturated, n–6 polyunsaturated, or n–3 polyunsaturated fats were provided during both low cholesterol and cholesterol-enriched stages; sera and HDL₃ samples were obtained at specific points during the treatment period. Analysis of the HDL phospholipid composition revealed significant acyl chain modification, consistent with the respective fat-specific diet. Cholesterol efflux from mouse L-cell fibroblasts to HDL₃ isolated from the specific diet groups was measured and revealed no differences in the abilities of the particles to accept cellular cholesterol; determination of the bidirectional flux of cholesterol between the cells and HDL₃ species further demonstrated no effect of phospholipid acyl chain modification on this process. The effects of dietary modification of phospholipid acyl chains on cellular cholesterol efflux were directly examined by isolating the HDL phospholipid and combining it with human apolipoprotein A-I to form well-defined reconstituted HDL particles. These complexes did not display any differences with respect to their ability to stimulate cellular cholesterol efflux. Incubations with 5% sera further confirmed that the fat-specific diets do not influence cholesterol efflux.

These results suggest that the established influences of specific dietary fats on the progression of atherosclerosis are due to effects on cholesterol metabolism other than the efflux of cellular cholesterol in the first step of reverse cholesterol transport.—Gillotte, K. L., S. Lund-Katz, M. de la Llera-Moya, J. S. Parks, L. L. Rudel, G. H. Rothblat, and M. C. Phillips. Dietary modification of high density lipoprotein phospholipid and influence on cellular cholesterol efflux. J. Lipid Res. 1998. 39: 2065–2075.

Supplementary key words: acyl chain, monounsaturated, saturated, polyunsaturated

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				V. Buonacorso, E. R Nakandakare, V. S Nunes, M. Passarelli, E. C. Quintao, and A. M. P Lottenberg Macrophage cholesterol efflux elicited by human total plasma and by HDL subfractions is not affected by different types of dietary fatty acids Am. J. Clinical Nutrition, November 1, 2007; 86(5): 1270 - 1277. [Abstract] [Full Text] [PDF]

				N. R. Matthan, F. K. Welty, P. H. R. Barrett, C. Harausz, G. G. Dolnikowski, J. S. Parks, R. H. Eckel, E. J. Schaefer, and A. H. Lichtenstein Dietary Hydrogenated Fat Increases High-Density Lipoprotein apoA-I Catabolism and Decreases Low-Density Lipoprotein apoB-100 Catabolism in Hypercholesterolemic Women Arterioscler. Thromb. Vasc. Biol., June 1, 2004; 24(6): 1092 - 1097. [Abstract] [Full Text]

				P. A. Dawson, J. Haywood, A. L. Craddock, M. Wilson, M. Tietjen, K. Kluckman, N. Maeda, and J. S. Parks Targeted Deletion of the Ileal Bile Acid Transporter Eliminates Enterohepatic Cycling of Bile Acids in Mice J. Biol. Chem., September 5, 2003; 278(36): 33920 - 33927. [Abstract] [Full Text] [PDF]

				A. T. Lada, L. L. Rudel, and R. W. St. Clair Effects of LDL enriched with different dietary fatty acids on cholesteryl ester accumulation and turnover in THP-1 macrophages J. Lipid Res., April 1, 2003; 44(4): 770 - 779. [Abstract] [Full Text] [PDF]

				L. M. Gatto, M. A. Lyons, A. J. Brown, and S. Samman Trans Fatty Acids Affect Lipoprotein Metabolism in Rats J. Nutr., June 1, 2002; 132(6): 1242 - 1248. [Abstract] [Full Text] [PDF]

				M. T. Montoya, A. Porres, S. Serrano, J. C. Fruchart, P. Mata, J. A. G. Gerique, and G. R. Castro Fatty acid saturation of the diet and plasma lipid concentrations, lipoprotein particle concentrations, and cholesterol efflux capacity Am. J. Clinical Nutrition, March 1, 2002; 75(3): 484 - 491. [Abstract] [Full Text] [PDF]

				J. W. Furbee Jr., O. Francone, and J. S. Parks Alteration of plasma HDL cholesteryl ester composition with transgenic expression of a point mutation (E149A) of human LCAT J. Lipid Res., October 1, 2001; 42(10): 1626 - 1635. [Abstract] [Full Text] [PDF]

				K. W. Huggins, P. L. Colvin, E. R. Burleson, K. Kelley, J. K. Sawyer, P. H. R. Barrett, L. L. Rudel, and J. S. Parks Dietary n-3 polyunsaturated fat increases the fractional catabolic rate of medium-sized HDL particles in African green monkeys J. Lipid Res., September 1, 2001; 42(9): 1457 - 1466. [Abstract] [Full Text] [PDF]