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The Journal of Lipid Research, Vol. 39, 2086-2090, October 1998
Copyright © 1998 by Lipid Research, Inc.

Article on Methodology

A comparison of LDL size determination using gradient gel electrophoresis and light-scattering methods

Correspondence to: J. D. Best.

This study compared gradient gel electrophoresis (GGE) and light-scattering (LS) methods of determining low density lipoprotein (LDL) particle size. LDL was isolated from 27 fasting subjects. Peak particle size was determined by GGE on 3–13% gradient gels (Gradipore, Sydney, Australia) and by LS using a Zetasizer 3000 (Malvern Instruments, Malvern, UK). Repeated measurements on a single specimen indicated a coefficient of variation (CV) of 0.3%. A correlation was noted (P < 0.0001; r = 0.78) when comparing LDL particle size determined by LS methodology and GGE.

Particle diameter results obtained by LS were smaller than those obtained by GGE (23.1 ± 0.1 vs. 26.1 ± 0.1 nm; P < 0.0001). LDL particle size determined by LS methodology correlated inversely with the log of triglyceride level (P < 0.0001; r = -0.77) and positively with high density lipoprotein (HDL) cholesterol level (P < 0.002; r = 0.57).—O'Neal, D., P. Harrip, G. Dragicevic, D. Rae, and J. D. Best. A comparison of LDL size determination using gradient gel electrophoresis and light-scattering methods. J. Lipid Res. 1998. 39: 2086–2090.

Supplementary key words: LDL size, light scattering, photon correlation spectroscopy, gradient gel electrophoresis

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