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The Journal of Lipid Research, Vol. 39, 2099-2105, October 1998
Copyright © 1998 by Lipid Research, Inc.

Article on Methodology

Simultaneous determination of homologues of vitamin E and coenzyme Q and products of -tocopherol oxidation

Correspondence to: Claude Leray.

A sensitive procedure is described for the simultaneous determination of vitamin E and coenzyme Q homologues and -tocopherol oxidation products using two-isocratic step high pressure liquid chromatography (HPLC) and electrochemical detection in the oxidative mode. Zinc-catalyzed reduction in a post-column reactor allows the detection of -tocopherolquinone, epoxy-tocopherolquinone, and ubiquinones. This technique was used to quantify lipophilic antioxidants in the liver tissue of rats treated or not with -tocopherolquinone and in a plant oil. -Tocopherolquinone and its epoxide derivatives, formed from -tocopherol during iron-catalyzed phospholipid peroxidation, were also determined in a liposome suspension. The high selectivity and sensitivity of the coulometric detection system enabled use of low oxidation potentials giving little baseline noise, while a fast isolation procedure and quantitative recoveries of all oxidized and reduced forms made it possible to measure a high ubiquinol/ubiquinone ratio in liver tissue. Administration of -tocopherolquinone to rats did not alter the antioxidant status of the liver, despite strong accumulation of both this quinone and its reduced form, -tocopherolhydroquinone.

These results indicate the presence of an efficient reductase and suggest that it could contribute to the protection of cellular membranes from oxidative stress.—Leray, C., M. D. Andriamampandry, M. Freund, C. Gachet, and J-P. Cazenave. Simultaneous determination of homologues of vitamin E and coenzyme Q and products of -tocopherol oxidation. J. Lipid Res. 1998. 39: 2099–2105.

Supplementary key words: HPLC, electrochemical detection, Zn catalyst reduction, ubiquinols, ubiquinones, tocopherols, tocotrienols

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