J. Lipid Res.  Neurobiology of Lipids (ISSN1683-5506)
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ecsedy, J. A.
Right arrow Articles by Seyfried, T. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ecsedy, J. A.
Right arrow Articles by Seyfried, T. N.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
The Journal of Lipid Research, Vol. 39, 2218-2227, November 1998
Copyright © 1998 by Lipid Research, Inc.


Original Article

Tumor-infiltrating macrophages influence the glycosphingolipid composition of murine brain tumors

Jeffrey A. Ecsedya, Herbert C. Yoheb,c, Alan J. Bergeronb, and Thomas N. Seyfrieda
a Department of Biology, Boston College, Chestnut Hill, MA, 02167-3811
b VA Medical and Regional Office Center, White River Junction, VT, 05009
c Department of Pharmacology, Dartmouth Medical School, Hanover, NH, 03755

Correspondence to: Thomas N. Seyfried.

A procedure was developed to analyze glycosphingolipids (GSLs) in tumor-infiltrating macrophages (TIMs). The procedure entailed dissociating tumors into single cell suspensions with a concurrent metabolic labeling of GSLs using [14C]galactose. TIMs were then separated from tumor cells and other host cells by magnetic activated cell sorting and CD11b (Mac-1) microbeads. Gangliosides and neutral glycosphingolipids were analyzed in the TIM-enriched and TIM-depleted fractions in two different murine brain tumors (EPEN and CT-2A). The TIM gangliosides consisted of over 30 structures as assessed by two-dimensional high performance thin-layer chromatography. GSLs enriched in TIMs, relative to the tumors, included Gg4Cer (asialo GM1), GM1b, and GD1{alpha}. TIM GSLs were similar in EPEN and CT-2A despite their differences in growth and morphology. TIM GSLs were similar whether TIMs were isolated from tumors grown intracranially or subcutaneously. TIM GSLs were also similar to activated peritoneal macrophage GSLs, although differences in the ceramide structure were observed. Knowledge of TIM GSLs will be important in determining the function of these molecules in macrophage–tumor interactions. In addition, these methods will be helpful in determining the cellular origin of human brain tumor GSLs and in identifying tumor-associated GSLs for immunotherapy.—Ecsedy, J. A., H. C. Yohe, A. J. Bergeron, and T. N. Seyfried. Tumor-infiltrating macrophages influence the glycosphingolipid composition of murine brain tumors. J. Lipid Res. 1998. 39: 2218–2227.

Supplementary key words: ganglioside, glycolipid, GM1b, GD1{alpha}, asialo GM1, macrophage, CD11b, Mac-1, cancer, astrocytoma, immunotherapy


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
GlycobiologyHome page
I. Popa, A. Pons, C. Mariller, T. Tai, J.-P. Zanetta, L. Thomas, and J. Portoukalian
Purification and structural characterization of de-N-acetylated form of GD3 ganglioside present in human melanoma tumors
Glycobiology, April 1, 2007; 17(4): 367 - 373.
[Abstract] [Full Text] [PDF]


Home page
Clin. Cancer Res.Home page
P. Mukherjee, L. E. Abate, and T. N. Seyfried
Antiangiogenic and Proapoptotic Effects of Dietary Restriction on Experimental Mouse and Human Brain Tumors
Clin. Cancer Res., August 15, 2004; 10(16): 5622 - 5629.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
M. Skoberne, S. Schenk, H. Hof, and G. Geginat
Cross-Presentation of Listeria monocytogenes-Derived CD4 T Cell Epitopes
J. Immunol., August 1, 2002; 169(3): 1410 - 1418.
[Abstract] [Full Text] [PDF]


Home page
J. Lipid Res.Home page
B. Garner, D. A. Priestman, R. Stocker, D. J. Harvey, T. D. Butters, and F. M. Platt
Increased glycosphingolipid levels in serum and aortae of apolipoprotein E gene knockout mice
J. Lipid Res., February 1, 2002; 43(2): 205 - 214.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
T. Okajima, H.-H. Chen, H. Ito, M. Kiso, T. Tai, K. Furukawa, T. Urano, and K. Furukawa
Molecular Cloning and Expression of Mouse GD1alpha /GT1aalpha /GQ1balpha Synthase (ST6GalNAc VI) Gene
J. Biol. Chem., March 15, 2000; 275(10): 6717 - 6723.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
T. Okajima, S. Fukumoto, H. Ito, M. Kiso, Y. Hirabayashi, T. Urano, K. Furukawa, and K. Furukawa
Molecular Cloning of Brain-specific GD1alpha Synthase (ST6GalNAc V) Containing CAG/Glutamine Repeats
J. Biol. Chem., October 22, 1999; 274(43): 30557 - 30562.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
M. G. Manfredi, S. Lim, K. P. Claffey, and T. N. Seyfried
Gangliosides Influence Angiogenesis in an Experimental Mouse Brain Tumor
Cancer Res., October 1, 1999; 59(20): 5392 - 5397.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 1998 by the American Society for Biochemistry and Molecular Biology.