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The Journal of Lipid Research, Vol. 39, 2319-2328, December 1998
Copyright © 1998 by Lipid Research, Inc.

Original Article

VLDL-triglyceride production after alcohol ingestion, studied using [2-¹³C₁] glycerol

Correspondence to: Marc K. Hellerstein.

We used [2-¹³C₁]glycerol to characterize very low density lipoprotein (VLDL)-triglyceride kinetics and intrahepatic glycerol metabolism in normal men (n = 4) after alcohol (EtOH) ingestion. [2-¹³C₁]glycerol was infused before and after the consumption of 48 g EtOH or a placebo. Three additional subjects also received [1-¹³C₁]acetate in addition to the [2-¹³C₁]glycerol with EtOH treatment. Incorporation of tracer into the glycerol or fatty acid moiety of VLDL-triglyceride was measured by gas chromatography–mass spectrometry and used to calculate VLDL-triglyceride production rates. Intrahepatic triose-phosphate enrichments were also calculated based on mass isotopomer distribution analysis of plasma glucose. There was no difference in VLDL-triglyceride production rates after 48 g EtOH (11.9 ± 3.7 mg/kg/h) or placebo (14.7 ± 3.3 mg/kg/h). The VLDL-triglyceride rate constants calculated by kinetic modeling using the glycerol and acetate tracers in the combined isotope infusion subjects were very closely correlated (r ² = 0.94). The peak VLDL-glycerol enrichments after EtOH were 22.5 ± 3.3% versus 7.6 ± 0.8% after placebo (P < 0.001), while intrahepatic triose-phosphate enrichments were 19.8 ± 1.3% and 13.1 ± 1.2% (P < 0.001), respectively. Moreover, the calculated asymptotic VLDL-glycerol enrichments (representing the hepatic -glycerol phosphate enrichment) were significantly higher after EtOH than placebo. The higher ratio of VLDL-glycerol to triose-phosphate labeling after EtOH suggests a metabolic block at glycerol 3-phosphate dehydrogenase.

We conclude that consumption of 48 g EtOH does not increase VLDL-triglyceride production in normal men but does cause accumulation of tracer in hepatic -glycerol phosphate.—Siler, S. Q., R. A. Neese, E. J. Parks, and M. K. Hellerstein. VLDL-triglyceride production after alcohol ingestion studied using [2-¹³C₁]glycerol. J. Lipid Res. 1998. 39: 2319–2328.

Supplementary key words: MIDA, alcohol, glycerol phosphate, hypertriglyceridemia, kinetic modeling

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