HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Martins, I. J. Articles by Redgrave, T. G. Search for Related Content PubMed PubMed Citation Articles by Martins, I. J. Articles by Redgrave, T. G. Social Bookmarking                      What's this?

The Journal of Lipid Research, Vol. 39, 302-312, February 1998
Copyright © 1998 by Lipid Research, Inc.

Original Article

Sterol side chain length and structure affect the clearance of chylomicron-like lipid emulsions in rats and mice

Correspondence to: Ian J. Martins.

In previous work we found that sterols such as cholesterol were essential for physiological plasma clearance of lipid emulsions mimicking the structure of mammalian triglyceride-rich lipoproteins. In the present study we compared the clearances of emulsions prepared with sterols of varying alkyl chain length (straight chains, n –C3 to n –C7, or branched chains, i –C5 to i –C10) at the C-17 position. Our studies show that the length of the alkyl chain at the C-17 position of sterols markedly affects the removal of remnant particles from the plasma of rats traced by emulsion cholesteryl oleate label. An alkyl chain of 7 carbons or more was needed for normal remnant clearance. Straight and branched chains of similar length were cleared similarly, showing that the presence of a branch at the end of the alkyl chain had no effect on remnant clearance. For side chains of 7 carbons or less, substitution of sterols with an unsaturation in the alkyl chain close to the terminal carbon markedly decreased the clearance of remnants. Triolein label was used to estimate lipolysis of the injected emulsions. Lipolysis was little affected by the structure of the sterol side chain, except that lipolysis was markedly higher with emulsions containing sterols with an alkyl chain having 4 carbon atoms (n –C4) or with an unsaturation in the 4 carbon alkyl chain.

We conclude that the length of the alkyl side chain is an important element in the essentiality of cholesterol as a regulator of metabolism of lipid emulsion models of triglyceride-rich lipoproteins.—Martins, I. J., C. Vilchèze, B-C. Mortimer, R. Bittman, and T. G. Redgrave. Sterol side chain length and structure affect the clearance of chylomicron-like lipid emulsions in rats and mice. J. Lipid Res. 1998. 39: 302–312.

Supplementary key words: sterol, alkyl chain, emulsion, cholesteryl oleate, triolein, lipolysis, clearance, rats, remnants, apoE knockout mice

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				F. Behzad Kasravi, D. H. Lee, K. Weisgraber, and H. W. Harris Lipoprotein-bound endotoxin exerts an immunomodulatory effect on hepatocytes through the lipid A domain of LPS Innate Immunity, February 1, 2005; 11(1): 19 - 24. [Abstract] [PDF]

				D. C. Chan, G. F. Watts, P. H. R. Barrett, I. J. Martins, A. P. James, J. C. L. Mamo, T. A. Mori, and T. G. Redgrave Effect of atorvastatin on chylomicron remnant metabolism in visceral obesity: a study employing a new stable isotope breath test J. Lipid Res., May 1, 2002; 43(5): 706 - 712. [Abstract] [Full Text] [PDF]

				I. J. Martins, E. Hone, C. Chi, U. Seydel, R. N. Martins, and T. G. Redgrave Relative roles of LDLr and LRP in the metabolism of chylomicron remnants in genetically manipulated mice J. Lipid Res., February 1, 2000; 41(2): 205 - 213. [Abstract] [Full Text]

				B.-J. Zeng, B.-C. Mortimer, I. J. Martins, U. Seydel, and T. G. Redgrave Chylomicron remnant uptake is regulated by the expression and function of heparan sulfate proteoglycan in hepatocytes J. Lipid Res., April 1, 1998; 39(4): 845 - 860. [Abstract] [Full Text]