J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Neuzil, J.
Right arrow Articles by Stocker, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Neuzil, J.
Right arrow Articles by Stocker, R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

The Journal of Lipid Research, Vol. 39, 354-368, February 1998
Copyright © 1998 by Lipid Research, Inc.

Original Article

Radical-induced lipoprotein and plasma lipid oxidation in normal and apolipoprotein E gene knockout (apoE-/-) mice: apoE-/- mouse as a model for testing the role of tocopherol-mediated peroxidation in atherogenesis

Jirí Neuzila, Julie K. Christisona, Eugene Iheanachob, Jean-Charles Fragonasb, Vivienne Zammita, Nicholas H. Huntb, and Roland Stockera
a Biochemistry Unit, Heart Research Institute, 145 Missenden Road, Camperdown, NSW 2050, Australia
b Department of Pathology, University of Sydney, Sydney, NSW 2006, Australia

Correspondence to: Roland Stocker.

Exposure of plasma from apolipoprotein E gene knockout (apoE-/-) and control (CBA or C57BL/6J) mice plasma to a constant rate of aqueous peroxyl radicals (ROO .) resulted in the depletion of ascorbate, urate and {alpha}-tocopherol ({alpha}-TOH), with substantial and little lipid peroxidation, respectively. {alpha}-TOH levels were 3-times higher in plasma from apoE-/- than control mice and its addition enhanced the oxidizability of control mouse plasma. In apoE-/- mouse plasma, {alpha}-TOH was associated primarily with very low density lipoprotein (VLDL), whereas in plasma from control mice, the vitamin was located largely in high density lipoproteins. Oxidation of isolated lipoproteins by ROO . resulted in the accumulation of lipid hydroperoxides to an extent that reflected the plasma concentration and {alpha}-TOH content of the different lipoprotein fractions. Oxidation of `plasma' reconstituted from components of apoE-/- mice and/or human plasma showed that human and apoE-/- mouse lipoproteins peroxidized with similar kinetics, although the initiation of lipid peroxidation was greater in the presence of mouse than human lipoprotein-deficient plasma. Also, the chain length of lipid peroxidation in apoE-/- mouse plasma after ascorbate depletion appeared to be independent of the rate of ROO . generation.

Together, these results show that the ROO . induced peroxidation of plasma lipoproteins in atherogenesis-susceptible apoE-/- mice exhibits some, though not all, features of tocopherol-mediated peroxidation (TMP). Therefore, apoE-/- mice may represent a suitable animal model to test a role for TMP in atherogenesis and the prevention of this disease by anti-TMP agents.—Neuzil, J., J. K. Christison, E. Iheanacho, J-C. Fragonas, V. Zammit, N. H. Hunt, and R. Stocker. Radical-induced lipoprotein and plasma lipid oxidation in normal and apolipoprotein E gene knockout (apoE-/-) mice: apoE-/- mouse as a model for testing the role of tocopherol-mediated peroxidation in atherogenesis. J. Lipid Res. 1998. 39: 354–368.

Supplementary key words: antioxidants, atherosclerosis, lipid peroxidation, vitamin E


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Exp. Med.Home page
A. T. Shamshiev, F. Ampenberger, B. Ernst, L. Rohrer, B. J. Marsland, and M. Kopf
Dyslipidemia inhibits Toll-like receptor-induced activation of CD8{alpha}-negative dendritic cells and protective Th1 type immunity
J. Exp. Med., February 19, 2007; 204(2): 441 - 452.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
F.-S. Shie, M. D. Neely, I. Maezawa, H. Wu, S. J. Olson, G. Jurgens, K. S. Montine, and T. J. Montine
Oxidized Low-Density Lipoprotein Is Present in Astrocytes Surrounding Cerebral Infarcts and Stimulates Astrocyte Interleukin-6 Secretion
Am. J. Pathol., April 1, 2004; 164(4): 1173 - 1181.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
R. Stocker and R. A O'Halloran
Dealcoholized red wine decreases atherosclerosis in apolipoprotein E gene-deficient mice independently of inhibition of lipid peroxidation in the artery wall
Am. J. Clinical Nutrition, January 1, 2004; 79(1): 123 - 130.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. M. Troen, E. Lutgens, D. E. Smith, I. H. Rosenberg, and J. Selhub
The atherogenic effect of excess methionine intake
PNAS, December 9, 2003; 100(25): 15089 - 15094.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
P. Mardones, P. Strobel, S. Miranda, F. Leighton, V. Quinones, L. Amigo, J. Rozowski, M. Krieger, and A. Rigotti
{alpha}-Tocopherol Metabolism Is Abnormal in Scavenger Receptor Class B Type I (SR-BI)-Deficient Mice
J. Nutr., March 1, 2002; 132(3): 443 - 449.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
B. Garner, D. A. Priestman, R. Stocker, D. J. Harvey, T. D. Butters, and F. M. Platt
Increased glycosphingolipid levels in serum and aortae of apolipoprotein E gene knockout mice
J. Lipid Res., February 1, 2002; 43(2): 205 - 214.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
S. R. Thomas, S. B. Leichtweis, K. Pettersson, K. D. Croft, T. A. Mori, A. J. Brown, and R. Stocker
Dietary Cosupplementation With Vitamin E and Coenzyme Q10 Inhibits Atherosclerosis in Apolipoprotein E Gene Knockout Mice
Arterioscler. Thromb. Vasc. Biol., April 1, 2001; 21(4): 585 - 593.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
X. Niu, V. Zammit, J. M. Upston, R. T. Dean, and R. Stocker
Coexistence of Oxidized Lipids and {alpha}-Tocopherol in All Lipoprotein Density Fractions Isolated From Advanced Human Atherosclerotic Plaques
Arterioscler. Thromb. Vasc. Biol., July 1, 1999; 19(7): 1708 - 1718.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
J. M. Letters, P. K. Witting, J. K. Christison, A. W. Eriksson, K. Pettersson, and R. Stocker
Time-dependent changes to lipids and antioxidants in plasma and aortas of apolipoprotein E knockout mice
J. Lipid Res., June 1, 1999; 40(6): 1104 - 1112.
[Abstract] [Full Text]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
A. Shaish, J. George, B. Gilburd, P. Keren, H. Levkovitz, and D. Harats
Dietary ß-Carotene and {alpha}-Tocopherol Combination Does Not Inhibit Atherogenesis in an ApoE–Deficient Mouse Model
Arterioscler. Thromb. Vasc. Biol., June 1, 1999; 19(6): 1470 - 1475.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
P. K. WITTING, K. PETTERSSON, A.-M. ÖSTLUND-LINDQVIST, C. WESTERLUND, A. W. ERIKSSON, and R. STOCKER
Inhibition by a coantioxidant of aortic lipoprotein lipid peroxidation and atherosclerosis in apolipoprotein E and low density lipoprotein receptor gene double knockout mice
FASEB J, April 1, 1999; 13(6): 667 - 675.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 1998 by the American Society for Biochemistry and Molecular Biology.