HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Eckhardt, E. R. M. Articles by VanBerge-Henegouwen, G. P. Search for Related Content PubMed PubMed Citation Articles by Eckhardt, E. R. M. Articles by VanBerge-Henegouwen, G. P. Social Bookmarking                      What's this?

The Journal of Lipid Research, Vol. 39, 594-603, March 1998
Copyright © 1998 by Lipid Research, Inc.

Original Article

Quantitation of cholesterol-carrying particles in human gallbladder bile

Correspondence to: Erik R. M. Eckhardt.

The inter-mixed micellar/vesicular (non-phospholipid-associated) bile salt concentration (IMC) can be rapidly measured in model biles by centrifugal ultrafiltration, thus allowing reliable separation of vesicular and micellar cholesterol carriers by gel filtration with an elution buffer containing bile salts at the correct IMC (Donovan, J. M., and A. A. Jackson. 1993. J. Lipid Res. 34: 1121–1129). We adapted this method to the more complex human gallbladder bile and examined the relationship between cholesterol solubilization and crystallization in gallbladder biles from 10 cholesterol gallstone patients. The IMC (mean ± SEM) was 9.67 ± 1.97 (range 3.56 – 35.02) mM with significant enrichment with hydrophilic bile salt species. Upon gel filtration of these biles with an eluant buffer containing 10 major bile salts at concentrations according to their IMC, cholesterol was found to be solubilized mainly in mixed micelles. Vesicles were detected in all 10 biles after separation by KBr density gradient ultracentrifugation but in only 5 of these biles with the IMC method. Biles without vesicles had a lower CSI (1.15 ± 0.12 vs. 1.90 ± 0.28, P < 0.05), a higher total lipid concentration (11.9 ± 2.3 vs. 5.9 ± 1.1, P < 0.05), and a higher bile salt/(bile salt + phospholipid) ratio (0.83 ± 0.01 vs. 0.74 ± 0.04, P = 0.07). For both IMC and ultracentrifugation methods, vesicular cholesterol concentration showed a negative correlation with crystal observation time and a positive correlation with cumulative crystal score during 21 days.

Our data indicate that methods such as density gradient ultracentrifugation overestimate vesicular cholesterol solubilization in human biles. —Eckhardt, E. R. M., B. J. M. van de Heijning, K . J. van Erpecum, W. Renooij, and G. P. VanBerge-Henegouwen. Quantitation of cholesterol-carrying particles in human gallbladder bile. J. Lipid Res. 1998. 39: 594–603.

Supplementary key words: bile, bile salts, cholesterol, crystallization, gallstones, IMC, micelles, vesicles

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				R. K. Keller, M. Small, and S. J. Fliesler Enzyme blockade: a nonradioactive method to determine the absolute rate of cholesterol synthesis in the brain J. Lipid Res., October 1, 2004; 45(10): 1952 - 1957. [Abstract] [Full Text] [PDF]

				A. Moschetta, G. P. vanBerge-Henegouwen, P. Portincasa, G. Palasciano, and K. J. van Erpecum Cholesterol crystallization in model biles: effects of bile salt and phospholipid species composition J. Lipid Res., August 1, 2001; 42(8): 1273 - 1281. [Abstract] [Full Text] [PDF]

				M. Souidi, M. Combettes-Souverain, F. Milliat, E. R. Eckhardt, O. Audas, S. Dubrac, M. Parquet, J. Férézou, and C. Lutton Hamsters Predisposed to Sucrose-Induced Cholesterol Gallstones (LPN Strain) Are More Resistant to Excess Dietary Cholesterol than Hamsters That Are Not Sensitive to Cholelithiasis Induction J. Nutr., June 1, 2001; 131(6): 1803 - 1811. [Abstract] [Full Text]

				J. Férézou, M. Combettes-Souverain, M. Souidi, J. L. Smith, N. Boehler, F. Milliat, E. Eckhardt, G. Blanchard, M. Riottot, C. Sérougne, et al. Cholesterol, bile acid, and lipoprotein metabolism in two strains of hamster, one resistant, the other sensitive (LPN) to sucrose-induced cholelithiasis J. Lipid Res., December 1, 2000; 41(12): 2042 - 2054. [Abstract] [Full Text]

				A. Moschetta, G. P. vanBerge-Henegouwen, P. Portincasa, G. Palasciano, A. K. Groen, and K. J. van Erpecum Sphingomyelin exhibits greatly enhanced protection compared with egg yolk phosphatidylcholine against detergent bile salts J. Lipid Res., June 1, 2000; 41(6): 916 - 924. [Abstract] [Full Text]

				E. R. M. Eckhardt, A. Moschetta, W. Renooij, S. S. Goerdayal, G. P. van Berge-Henegouwen, and K. J. van Erpecum Asymmetric distribution of phosphatidylcholine and sphingomyelin between micellar and vesicular phases: potential implications for canalicular bile formation J. Lipid Res., November 1, 1999; 40(11): 2022 - 2033. [Abstract] [Full Text]