HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Innis-Whitehouse, W. Articles by Le, N.-A. Search for Related Content PubMed PubMed Citation Articles by Innis-Whitehouse, W. Articles by Le, N.-A. Social Bookmarking                      What's this?

The Journal of Lipid Research, Vol. 39, 679-690, March 1998
Copyright © 1998 by Lipid Research, Inc.

Paper on Methodology

An efficient chromatographic system for lipoprotein fractionation using whole plasma

Correspondence to: Ngoc-Anh Le.

We have validated a semi-automatic procedure for the efficient isolation of plasma lipoproteins from 300 µl of whole plasma (actual injection volume 200 µl) by Fast Phase Liquid Chromatography (FPLC). Modified enzymatic assays were established to allow the determination of low concentrations (1–20 mg/dl) of triglycerides and cholesterol using the Beckman CX-5 Autoanalyzer. The sum of the cholesterol contents in the fractions corresponding to low density (LDL) and high density lipoprotein (HDL) can be demonstrated to be highly correlated to values obtained with dextran sulfate/MgCl₂ precipitation for HDLc (slope = 0.98, r ² = 0.997) and ultracentrifugation (beta-quant) for LDLc (slope = 1.03, r ² = 0.988). Using pure lipoprotein fractions isolated by ultracentrifugation, linear ranges of detection for HDLc and HDL apoA-I were performed at 18–95 mg/dl and 59–262 mg/dl, respectively. The ranges for LDLc were 41–435 mg/dl and 21–280 mg/dl for LDL apoB. The mean (range) fractional standard deviations for quadruplicate runs for 15 individual plasma samples ranging widely in lipoprotein concentrations were 0.97 (0.29–2.86%) for LDLc (range: 101.5–258.5 mg/dl), 3.67 (0.62–14.11%) for HDLc (range: 27.1–85.1 mg/dl) and 2.19 (0.16–6.56%) for VLDL-TG (range: 6.1–515.0 mg/dl).—Innis-Whitehouse, W., X. Li, W. V. Brown, and N-A. Le. An efficient chromatographic system for lipoprotein fractionation using whole plasma. J. Lipid Res. 1998. 39: 679–690.

Supplementary key words: fast phase liquid chromatography (FPLC), lipid distribution, apolipoprotein distribution

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. M. Jerico, F. De Camargo Chiquito, K. Kajihara, M. A. B. Moreira, R. Gonzales, F. L. A. Machado, V. S. Nunes, S. Catanozi, and E. R. Nakandakare Chromatographic analysis of lipid fractions in healthy dogs and dogs with obesity or hyperadrenocorticism J Vet Diagn Invest, March 1, 2009; 21(2): 203 - 207. [Abstract] [Full Text] [PDF]

				P. Wiesner, K. Leidl, A. Boettcher, G. Schmitz, and G. Liebisch Lipid profiling of FPLC-separated lipoprotein fractions by electrospray ionization tandem mass spectrometry J. Lipid Res., March 1, 2009; 50(3): 574 - 585. [Abstract] [Full Text] [PDF]

				T. Porsgaard, X. Xu, and H. Mu The Form of Dietary Conjugated Linoleic Acid Does Not Influence Plasma and Liver Triacylglycerol Concentrations in Syrian Golden Hamsters J. Nutr., August 1, 2006; 136(8): 2201 - 2206. [Abstract] [Full Text] [PDF]

				I. Giannopoulou, L. L. Ploutz-Snyder, R. Carhart, R. S. Weinstock, B. Fernhall, S. Goulopoulou, and J. A. Kanaley Exercise Is Required for Visceral Fat Loss in Postmenopausal Women with Type 2 Diabetes J. Clin. Endocrinol. Metab., March 1, 2005; 90(3): 1511 - 1518. [Abstract] [Full Text] [PDF]

				C. L. Sundell, P. K. Somers, C. Q. Meng, L. K. Hoong, K.-L. Suen, R. R. Hill, L. K. Landers, A. Chapman, D. Butteiger, M. Jones, et al. AGI-1067: A Multifunctional Phenolic Antioxidant, Lipid Modulator, Anti-Inflammatory and Antiatherosclerotic Agent J. Pharmacol. Exp. Ther., June 1, 2003; 305(3): 1116 - 1123. [Abstract] [Full Text] [PDF]

				M. Chetiveaux, H. Nazih, V. Ferchaud-Roucher, G. Lambert, Y. Zair, M. Masson, K. Ouguerram, D. Bouhours, and M. Krempf The differential apoA-I enrichment of pre{beta}1 and {alpha}HDL is detectable by gel filtration separation J. Lipid Res., November 1, 2002; 43(11): 1986 - 1993. [Abstract] [Full Text] [PDF]

				N. Khan-Merchant, M. Penumetcha, O. Meilhac, and S. Parthasarathy Oxidized Fatty Acids Promote Atherosclerosis Only in the Presence of Dietary Cholesterol in Low-Density Lipoprotein Receptor Knockout Mice J. Nutr., November 1, 2002; 132(11): 3256 - 3262. [Abstract] [Full Text] [PDF]

				K. Kobori, K. Saito, S. Ito, K. Kotani, M. Manabe, and T. Kanno A new enzyme-linked immunosorbent assay with two monoclonal antibodies to specific epitopes measures human lecithin-cholesterol acyltransferase J. Lipid Res., February 1, 2002; 43(2): 325 - 334. [Abstract] [Full Text] [PDF]

				S. Dithmar, N. A. Sharara, C. A. Curcio, N.-A. Le, Y. Zhang, S. Brown, and H. E. Grossniklaus Murine High-Fat Diet and Laser Photochemical Model of Basal Deposits in Bruch Membrane Arch Ophthalmol, November 1, 2001; 119(11): 1643 - 1649. [Abstract] [Full Text] [PDF]

				S. Dithmar, C. A. Curcio, N.-A. Le, S. Brown, and H. E. Grossniklaus Ultrastructural Changes in Bruch's Membrane of Apolipoprotein E-Deficient Mice Invest. Ophthalmol. Vis. Sci., July 1, 2000; 41(8): 2035 - 2042. [Abstract] [Full Text]

				E. D. Breyer, N.-A. Le, X. Li, D. Martinson, and W. V. Brown Apolipoprotein C-III displacement of apolipoprotein E from VLDL: effect of particle size J. Lipid Res., October 1, 1999; 40(10): 1875 - 1882. [Abstract] [Full Text]