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The Journal of Lipid Research, Vol. 39, 1106-1110, May 1998
Copyright © 1998 by Lipid Research, Inc.

Paper on Methodology

Measurement of plasma glycerol specific activity by high performance liquid chromatography to determine glycerol flux

Robert L. Judda, Rita Nelsona, Samuel Kleinb, Michael D. Jensena, and John M. Milesa
a Endocrine Research Unit, Mayo Foundation, Rochester, MN 55905
b Division of Gastroenterology, Washington University School of Medicine, St. Louis, MO 63110

Correspondence to: John M. Miles.

Previous methods for measuring plasma glycerol specific activity (SA) are suboptimal, making the determination of glycerol kinetics in vivo with radiotracers difficult. A new high performance liquid chromatography (HPLC) method is described that permits the accurate and specific measurement of glycerol SA. The method involves isolation of glycerol from plasma and the formation of a tribenzoyl derivative. Glycerol rate of appearance was measured in five human volunteers using both [2-3H]glycerol and [2H5] glycerol. There was close agreement between the glycerol appearance rates measured using the two approaches (1.66 ± 0.14 vs. 1.70 ± 0.10 µmol · kg-1 · min-1, respectively, P = NS). This HPLC method offers improved specificity over existing methods of measuring glycerol turnover using radiotracers.—Judd, R. L., R. Nelson, S. Klein, M. D. Jensen, and J. M. Miles. Measurement of plasma glycerol specific activity by high performance liquid chromatography to determine glycerol flux. J. Lipid Res. 1998. 39: 1106–1110.

Supplementary key words: lipolysis, HPLC, stable isotopes, radioisotopes


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