|
 |
|
|||||||
|
|||||||||
Correspondence to:
Thomas N. Tulenko.
Small angle X-ray diffraction was used to examine arterial smooth muscle cell (SMC) plasma membranes isolated from control and cholesterol-fed (2%) atherosclerotic rabbits. A microsomal membrane enriched with plasma membrane obtained from animals fed cholesterol for up to 13 weeks showed a progressive elevation in the membrane unesterified (free) cholesterol:phospholipid (C/PL) mole ratio. Beyond 9 weeks of cholesterol feeding, X-ray diffraction patterns demonstrated a lateral immiscible cholesterol domain at 37°C with a unit cell periodicity of 34 Å coexisting within the liquid crystalline lipid bilayer. On warming, the immiscible cholesterol domain disappeared, and on cooling it reappeared, indicating that the immiscible cholesterol domain was fully reversible. These effects were reproduced in a model C/PL binary lipid system. In rabbits fed cholesterol for less than 9 weeks, lesser increases in membrane C/PL mole ratio were observed. X-ray diffraction analysis demonstrated an increase in membrane bilayer width that correlated with the C/PL mole ratio. This effect was also reproduced in a C/PL binary lipid system.
Taken together, these findings demonstrate that in vivo, feeding of cholesterol causes cholesterol–phospholipid interactions in the membrane bilayer that alter bilayer structure and organization. This interaction results in an increase in bilayer width peaking at a saturating membrane cholesterol concentration, beyond which lateral phase separation occurs resulting in the formation of separate cholesterol bilayer domains. These alterations in structure and organization in SMC plasma membranes may have significance in phenotypic modulation or aortic SMC during early atherogenesis.—Tulenko, T. N., M. Chen, P. E. Mason, and R. P. Mason. Physical effects of cholesterol on arterial smooth muscle membranes: evidence of immiscible cholesterol domains and alterations in bilayer width during atherogenesis. J. Lipid Res. 1998. 39: 947–956.
Supplementary key words:
X-ray diffraction, membrane structure, atherosclerosis, phospholipids, heart disease, vascular disease
Copyright © 1998 by Lipid Research, Inc.
Original Article
Physical effects of cholesterol on arterial smooth muscle membranes: evidence of immiscible cholesterol domains and alterations in bilayer width during atherogenesis
Thomas N. Tulenkoa,
Meng Chena,
Pamela E. Masonb, and
R. Preston Masonb
a Department of Physiology, Allegheny University of the Health Sciences, MCP · Hahnemann School of Medicine, 2900 Queen Lane, Philadelphia, PA 19129
b Neurosciences Research Center, Allegheny University of the Health Sciences, MCP · Hahnemann School of Medicine, Allegheny Campus, Pittsburgh, PA 15212-4772
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Nuotio, P. M. Isoviita, J. Saksi, P. Ijas, J. Pitkaniemi, R. Sonninen, L. Soinne, E. Saimanen, O. Salonen, P. T. Kovanen, et al. Adipophilin Expression Is Increased in Symptomatic Carotid Atherosclerosis: Correlation With Red Blood Cells and Cholesterol Crystals * Online Data Stroke, June 1, 2007; 38(6): 1791 - 1798. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Wustner Plasma Membrane Sterol Distribution Resembles the Surface Topography of Living Cells Mol. Biol. Cell, January 1, 2007; 18(1): 211 - 228. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Batova, J. DeWever, T. Godfraind, J.-L. Balligand, C. Dessy, and O. Feron The calcium channel blocker amlodipine promotes the unclamping of eNOS from caveolin in endothelial cells Cardiovasc Res, August 1, 2006; 71(3): 478 - 485. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. P. Mason, M. F. Walter, C. A. Day, and R. F. Jacob Active Metabolite of Atorvastatin Inhibits Membrane Cholesterol Domain Formation by an Antioxidant Mechanism J. Biol. Chem., April 7, 2006; 281(14): 9337 - 9345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. F. Jacob and R. P. Mason Lipid Peroxidation Induces Cholesterol Domain Formation in Model Membranes J. Biol. Chem., November 25, 2005; 280(47): 39380 - 39387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Virmani, F. D. Kolodgie, A. P. Burke, A. V. Finn, H. K. Gold, T. N. Tulenko, S. P. Wrenn, and J. Narula Atherosclerotic Plaque Progression and Vulnerability to Rupture: Angiogenesis as a Source of Intraplaque Hemorrhage Arterioscler. Thromb. Vasc. Biol., October 1, 2005; 25(10): 2054 - 2061. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. B. Kahn, K. Boesze-Battaglia, D. W. Stepp, A. Petrov, Y. Huang, R. P. Mason, and T. N. Tulenko Influence of serum cholesterol on atherogenesis and intimal hyperplasia after angioplasty: inhibition by amlodipine Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H591 - H600. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. Walter, R. F. Jacob, B. Jeffers, M. M. Ghadanfar, G. M. Preston, J. Buch, and R. P. Mason Serum levels of thiobarbituric acid reactive substances predict cardiovascular events in patients with stable coronary artery disease: A longitudinal analysis of the PREVENT study J. Am. Coll. Cardiol., November 16, 2004; 44(10): 1996 - 2002. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. K. Bowles, C. L. Heaps, J. R. Turk, K. K. Maddali, and E. M. Price Hypercholesterolemia inhibits L-type calcium current in coronary macro-, not microcirculation J Appl Physiol, June 1, 2004; 96(6): 2240 - 2248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. P. Mason, M. F. Walter, and R. F. Jacob Effects of HMG-CoA Reductase Inhibitors on Endothelial Function: Role of Microdomains and Oxidative Stress Circulation, June 1, 2004; 109(21_suppl_1): II-34 - II-41. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Pouvreau, C. Berthier, S. Blaineau, J. Amsellem, R. Coronado, and C. Strube Membrane cholesterol modulates dihydropyridine receptor function in mice fetal skeletal muscle cells J. Physiol., March 1, 2004; 555(2): 365 - 381. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Huang, K.E. Walker, F. Hanley, J. Narula, S.R. Houser, and T.N. Tulenko Cardiac Systolic and Diastolic Dysfunction After a Cholesterol-Rich Diet Circulation, January 6, 2004; 109(1): 97 - 102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. D. Kolodgie, H. K. Gold, A. P. Burke, D. R. Fowler, H. S. Kruth, D. K. Weber, A. Farb, L.J. Guerrero, M. Hayase, R. Kutys, et al. Intraplaque Hemorrhage and Progression of Coronary Atheroma N. Engl. J. Med., December 11, 2003; 349(24): 2316 - 2325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R.P. Mason, P. Marche, and T.H. Hintze Novel Vascular Biology of Third-Generation L-Type Calcium Channel Antagonists: Ancillary Actions of Amlodipine Arterioscler. Thromb. Vasc. Biol., December 1, 2003; 23(12): 2155 - 2163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. P. Mason and R. F. Jacob Membrane Microdomains and Vascular Biology: Emerging Role in Atherogenesis Circulation, May 6, 2003; 107(17): 2270 - 2273. [Full Text] [PDF]
|
|
|
|
|
|
A. L. McIntosh, A. M. Gallegos, B. P. Atshaves, S. M. Storey, D. Kannoju, and F. Schroeder Fluorescence and Multiphoton Imaging Resolve Unique Structural Forms of Sterol in Membranes of Living Cells J. Biol. Chem., February 14, 2003; 278(8): 6384 - 6403. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Chao, M. Zhou, A. McIntosh, F. Schroeder, and A. B. Kier ACBP and cholesterol differentially alter fatty acyl CoA utilization by microsomal ACAT J. Lipid Res., January 1, 2003; 44(1): 72 - 83. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mori, H. Itabe, Y. Higashi, Y. Fujimoto, M. Shiomi, M. Yoshizumi, Y. Ouchi, and T. Takano Foam cell formation containing lipid droplets enriched with free cholesterol by hyperlipidemic serum J. Lipid Res., November 1, 2001; 42(11): 1771 - 1781. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Schroeder, A. M. Gallegos, B. P. Atshaves, S. M. Storey, A. L. McIntosh, A. D. Petrescu, H. Huang, O. Starodub, H. Chao, H. Yang, et al. Recent Advances in Membrane Microdomains: Rafts, Caveolae, and Intracellular Cholesterol Trafficking Experimental Biology and Medicine, November 1, 2001; 226(10): 873 - 890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. S. Kruth, I. Ifrim, J. Chang, L. Addadi, D. Perl-Treves, and W.-Y. Zhang Monoclonal antibody detection of plasma membrane cholesterol microdomains responsive to cholesterol trafficking J. Lipid Res., September 1, 2001; 42(9): 1492 - 1500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. F. Jacob, R. J. Cenedella, and R. P. Mason Direct Evidence for Immiscible Cholesterol Domains in Human Ocular Lens Fiber Cell Plasma Membranes J. Biol. Chem., October 29, 1999; 274(44): 31613 - 31618. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Kellner-Weibel, P. G. Yancey, W. G. Jerome, T. Walser, R. P. Mason, M. C. Phillips, and G. H. Rothblat Crystallization of Free Cholesterol in Model Macrophage Foam Cells Arterioscler. Thromb. Vasc. Biol., August 1, 1999; 19(8): 1891 - 1898. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. F. Jacob, R. J. Cenedella, and R. P. Mason Evidence for Distinct Cholesterol Domains in Fiber Cell Membranes from Cataractous Human Lenses J. Biol. Chem., April 20, 2001; 276(17): 13573 - 13578. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |