Original Article

13-Hydroxy octadecadienoic acid (13-HODE) inhibits triacylglycerol-rich lipoprotein secretion by CaCo-2 cells

Correspondence to: Shubha Murthy.

Oxidized lipids present in atherogenic lipoproteins are derived, in part, from the diet. To address the effects of an oxidized lipid on intestinal lipoprotein assembly and secretion, CaCo-2 cells were incubated with 13-HODE or its native fatty acid, linoleic acid, and triacylglycerol-rich lipoprotein synthesis and secretion were investigated. 13-HODE was readily taken up by cells and esterified to lipids. Although both fatty acids were largely esterified to neutral lipids, in comparison to neutral lipids containing linoleic acid, a greater proportion of cellular neutral lipids containing 13-HODE and/or its metabolites was secreted. Compared to linoleic acid, however, 13-HODE caused less triacylglycerol, derived from de novo synthesis, and less triacylglycerol mass to be secreted. Cells incubated with both linoleic acid and 13-HODE together secreted less triacylglycerol mass than did cells incubated with linoleic acid alone. Less newly synthesized apoB and apoB mass were secreted by cells incubated with 13-HODE without altering the abundance of apoB mRNA.

The fraction of newly synthesized apoB translocated into the secretory pathway of cells exposed to 13-HODE was significantly less than that observed in cells incubated with linolenic acid, suggesting that 13-HODE interfered with the assembly and secretion of triacylglycerol-rich lipoprotein particles.—Murthy, S., E. Born, S. Mathur, and F. J. Field. 13-Hydroxy octadecadienoic acid (13-HODE) inhibits triacylglycerol-rich lipoprotein secretion by CaCo-2 cells. J. Lipid Res. 1998. 39: 1254–1262.

Supplementary key words: 13-HODE, triacylglycerol, apoB, CaCo-2 cells

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. W. Newman, G. A. Kaysen, B. D. Hammock, and G. C. Shearer Proteinuria increases oxylipid concentrations in VLDL and HDL but not LDL particles in the rat J. Lipid Res., August 1, 2007; 48(8): 1792 - 1800. [Abstract] [Full Text] [PDF]

				R. Rivabene, M. Napolitano, A. Cantafora, and E. Bravo Redox-Dependent Modulation of Lipid Synthesis Induced by Oleic Acid in the Human Intestinal Epithelial Cell LineCaco-2 Experimental Biology and Medicine, March 1, 2001; 226(3): 191 - 198. [Abstract] [Full Text]

				F. Courtois, I. Suc, C. Garofalo, M. Ledoux, E. Seidman, and E. Levy Iron-ascorbate alters the efficiency of Caco-2 cells to assemble and secrete lipoproteins Am J Physiol Gastrointest Liver Physiol, July 1, 2000; 279(1): G12 - G19. [Abstract] [Full Text] [PDF]