|
The Journal of Lipid Research, Vol. 39, 1254-1262, June 1998
Copyright © 1998 by Lipid Research, Inc.
13-Hydroxy octadecadienoic acid (13-HODE) inhibits triacylglycerol-rich lipoprotein secretion by CaCo-2 cells
Shubha Murthya,
Ella Borna,
Satya Mathura, and
F. Jeffrey Fielda
a Department of Internal Medicine, College of Medicine, University of Iowa, Iowa City, IA 52242
Correspondence to:
Shubha Murthy.
Oxidized lipids present in atherogenic lipoproteins are derived, in part, from the diet. To address the effects of an oxidized lipid on intestinal lipoprotein assembly and secretion, CaCo-2 cells were incubated with 13-HODE or its native fatty acid, linoleic acid, and triacylglycerol-rich lipoprotein synthesis and secretion were investigated. 13-HODE was readily taken up by cells and esterified to lipids. Although both fatty acids were largely esterified to neutral lipids, in comparison to neutral lipids containing linoleic acid, a greater proportion of cellular neutral lipids containing 13-HODE and/or its metabolites was secreted. Compared to linoleic acid, however, 13-HODE caused less triacylglycerol, derived from de novo synthesis, and less triacylglycerol mass to be secreted. Cells incubated with both linoleic acid and 13-HODE together secreted less triacylglycerol mass than did cells incubated with linoleic acid alone. Less newly synthesized apoB and apoB mass were secreted by cells incubated with 13-HODE without altering the abundance of apoB mRNA.
The fraction of newly synthesized apoB translocated into the secretory pathway of cells exposed to 13-HODE was significantly less than that observed in cells incubated with linolenic acid, suggesting that 13-HODE interfered with the assembly and secretion of triacylglycerol-rich lipoprotein particles.Murthy, S., E. Born, S. Mathur, and F. J. Field. 13-Hydroxy octadecadienoic acid (13-HODE) inhibits triacylglycerol-rich lipoprotein secretion by CaCo-2 cells. J. Lipid Res. 1998. 39: 12541262.
Supplementary key words:
13-HODE, triacylglycerol, apoB, CaCo-2 cells

CiteULike Complore Connotea Del.icio.us Digg Reddit Technorati What's this?
This article has been cited by other articles:

|
 |

|
 |
 
J. W. Newman, G. A. Kaysen, B. D. Hammock, and G. C. Shearer
Proteinuria increases oxylipid concentrations in VLDL and HDL but not LDL particles in the rat
J. Lipid Res.,
August 1, 2007;
48(8):
1792 - 1800.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. Rivabene, M. Napolitano, A. Cantafora, and E. Bravo
Redox-Dependent Modulation of Lipid Synthesis Induced by Oleic Acid in the Human Intestinal Epithelial Cell LineCaco-2
Experimental Biology and Medicine,
March 1, 2001;
226(3):
191 - 198.
[Abstract]
[Full Text]
|
 |
|

|
 |

|
 |
 
F. Courtois, I. Suc, C. Garofalo, M. Ledoux, E. Seidman, and E. Levy
Iron-ascorbate alters the efficiency of Caco-2 cells to assemble and secrete lipoproteins
Am J Physiol Gastrointest Liver Physiol,
July 1, 2000;
279(1):
G12 - G19.
[Abstract]
[Full Text]
[PDF]
|
 |
|
Copyright © 1998 by the American Society for Biochemistry and Molecular Biology.
|
Advertisement
Advertisement
|