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Correspondence to:
Laurence Wong.
Previously, we have shown that lipid-free apoA-I, when incubated with fibroblasts, will produce lipoproteins of pre-
We conclude that apoA-I may react with specific regions of plasma membrane to acquire this unusual lipid composition and that pre-
Supplementary key words:
high density lipoproteins, pre-alpha lipoproteins, pre-beta lipoproteins, fibroblasts, cholesterol, phospholipid composition, cholesterol efflux, two-dimensional electrophoresis
Copyright © 1998 by Lipid Research, Inc.
Original Article
Characterization of phospholipids in pre-
Wenwu Zhanga,
Bela Asztalosa,
Paul S. Roheima, and
Laurence Wonga
HDL: selective phospholipid efflux with apolipoprotein A-I
a Division of Lipoprotein Metabolism and Pathophysiology, Department of Physiology, Louisiana State University Medical Center, 1542 Tulane Avenue, New Orleans, LA 70112
mobility (Asztalos, B. F., et al. 1997. Arterioscler. Thromb. Vasc. Biol. 17: 16301636). In order to understand the nature of these pre-
particles, we further characterized their lipid content. The pre-
particles are high density lipoproteins, having a median density of 1.08 g/ml. They have a surface charge of -18.45 mV. The phospholipid composition of these particles showed that they have 4% each of phosphatidyl ethanolamine and inositol; 69% phosphatidyl choline and 18% sphingomyelin. This phospholipid composition is different from those of plasma HDL (81% phosphatidyl choline, 13% sphingomyelin), plasma membrane on the fibroblasts, and whole fibroblast phospholipid. To demonstrate that the pre-
mobility resides in the lipids, lipids from pre-
lipoproteins were reconstituted with lipid-free apoA-I. The resultant particles retained their pre-
mobility.
mobility is caused in part by the unusual phospholipid composition.Zhang, W., B. Asztalos, P. S. Roheim, and L. Wong. Characterization of phospholipids in pre-
HDL: selective phospholipid efflux with apolipoprotein A-I. J. Lipid Res. 1998. 39: 16011607. ![]()
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