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The Journal of Lipid Research, Vol. 39, 1601-1607, August 1998
Copyright © 1998 by Lipid Research, Inc.

Original Article

Characterization of phospholipids in pre- HDL: selective phospholipid efflux with apolipoprotein A-I

Correspondence to: Laurence Wong.

Previously, we have shown that lipid-free apoA-I, when incubated with fibroblasts, will produce lipoproteins of pre- mobility (Asztalos, B. F., et al. 1997. Arterioscler. Thromb. Vasc. Biol. 17: 1630–1636). In order to understand the nature of these pre- particles, we further characterized their lipid content. The pre- particles are high density lipoproteins, having a median density of 1.08 g/ml. They have a surface charge of -18.45 mV. The phospholipid composition of these particles showed that they have 4% each of phosphatidyl ethanolamine and inositol; 69% phosphatidyl choline and 18% sphingomyelin. This phospholipid composition is different from those of plasma HDL (81% phosphatidyl choline, 13% sphingomyelin), plasma membrane on the fibroblasts, and whole fibroblast phospholipid. To demonstrate that the pre- mobility resides in the lipids, lipids from pre- lipoproteins were reconstituted with lipid-free apoA-I. The resultant particles retained their pre- mobility.

We conclude that apoA-I may react with specific regions of plasma membrane to acquire this unusual lipid composition and that pre- mobility is caused in part by the unusual phospholipid composition.—Zhang, W., B. Asztalos, P. S. Roheim, and L. Wong. Characterization of phospholipids in pre- HDL: selective phospholipid efflux with apolipoprotein A-I. J. Lipid Res. 1998. 39: 1601–1607.

Supplementary key words: high density lipoproteins, pre-alpha lipoproteins, pre-beta lipoproteins, fibroblasts, cholesterol, phospholipid composition, cholesterol efflux, two-dimensional electrophoresis

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