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Journal of Lipid Research, Vol. 4, 130-138, April 1963
Copyright © 1963 by Lipid Research, Inc.

Microdetermination, isolation, and gas-liquid chromatography of 2-hydroxy fatty acids

Yasuo Kishimoto and Norman S. Radin

Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan

A new method is described for the specific isolation of 2-hydroxy fatty acids, particularly from brain lipids. The procedure involves saponification of the lipids with KOH-propylene glycol, preliminary purification with a small silica gel column, and precipitation as the copper chelate. The total content of 2-hydroxy acids may be determined by direct colorimetric determination of the copper in the precipitate, or the acids may be liberated and analyzed by gas-liquid chromatography. The acids are methylated with dimethoxypropane and acetylated with isopropenyl acetate prior to gas chromatographic analysis. The method is suitable for samples containing as little as 0.6 µmoles of hydroxy acid, but is readily scaled up for preparative isolations. An improved titrimetric procedure for copper is described.

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