Journal of Lipid Research, Vol. 4, 160-165, April 1963
Copyright © 1963 by Lipid Research, Inc.
Sterols of skin in the normal and triparanol-treated rat
L. Horlick and J. Avigan
Laboratory of Metabolism, National Heart Institute, National Institutes of Health, Bethesda 14, Maryland
Skin of normal rats and of rats treated with triparanol were incubated with acetate-2-H3 for 2 hr, and the sterols were extracted and separated chromatographically. Treatment with triparanol resulted in grossly visible changes (scaliness, roughness, and atrophy of the skin) and in a marked alteration in the metabolism of skin sterols. There were considerable reductions in the concentrations of compounds with saturated side chains: cholesterol, methostenol, and dihydrolanosterol; and increases in the concentrations of sterols with unsaturated side chains: desmosterol, lanosterol, and sterols tentatively identified as dehydro-methostenol and 
7,24-cholestadienol. Isotope data showed that after incubation for 2 hr, a large proportion of the counts were in squalene in skin from both normal and triparanol-treated rats. There was a reduction in the incorporation of radioactivity into the 5-stenol fraction and an increase in the incorporation into lanosterol and 7-sterols, in skin from triparanol-treated rats. Triparanol inhibits the enzyme responsible for reduction of the 24,25-double bond in the sterols of rat skin.
Submitted on September 24, 1962
Accepted on November 21, 1962
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