Journal of Lipid Research, Vol. 4, 166-178, April 1963
Copyright © 1963 by Lipid Research, Inc.
The skin sterols of normal and triparanol-treated rats
R. B. Clayton , Albert N. Nelson , and Ivan D. Frantz Jr.
Conant Chemical Laboratory, Harvard University, Cambridge 38, Massachusetts and Departments of Medicine and Physiological Chemistry, University of Minnesota Medical School, Minneapolis 14, Minnesota
The skin sterols of normal rats and of rats treated with the drug triparanol have been analyzed by means of chromatography on silicic acid followed by gas-liquid chromatography of the components of the peaks obtained from the silicic acid chromatogram. These two procedures complement each other in leading to complete separations of pairs of sterols that are poorly separated in either system alone. The results are shown to indicate the presence in rat skin of several previously undetected compounds for which molecular structures are proposed on the basis of the retention factor method. Evidence is presented for at least two effects of the drug triparanol, (1) in causing accumulation of the 
24-analogues of all the intermediates in cholesterol biosynthesis that normally occur in the 24,25-dihy-dro form, and (2) in causing marked alterations in the proportions of 7- to 8-isomers of these intermediates. The significance of these findings is discussed in the light of current views of the pathway of cholesterol biosynthesis.
Submitted on October 24, 1962
Accepted on January 23, 1963
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