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Journal of Lipid Research, Vol. 4, 260-265, July 1963
Copyright © 1963 by Lipid Research, Inc.

Isolation analysis of tissue fatty acids by ultramicro-ozonolysis in conjunction with thin-layer chromatography and gas-liquid chromatography

O. S. Privett , M. L. Blank , and O. Romanus

The Hormel Institute, University of Minnesota, Austin, Minnesota

Apparatus and technique for the detailed analysis of natural mixtures of fatty acids by a combination of thin-layer chromatography (TLC), gas-liquid chromatography (GLC), and reductive ozonolysis are described.

The fatty acids, as methyl esters, are separated on the basis of differences in unsaturation and chain length by the application of TLC using chromatoplates coated with silver nitrate-silica gel followed by GLC. Each group of fatty acid esters is analyzed by reductive ozonolysis by an ultramicromethod.

The general procedure is demonstrated with pure methyl esters and mixtures thereof, and it is applied to the analysis of the fatty acids of the liver phospholipids of rats fed a fat-free diet. The detection of endogenous 4,7,10,13-eicosatetraenoic acid of the palmitoleic acid family is reported.

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