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Journal of Lipid Research, Vol. 4, 402-406, October 1963
Copyright © 1963 by Lipid Research, Inc.

Purification and partial characterization of sphingomyelin from human plasma

Charles C. Sweeley

Department of Biochemistry and Nutrition, Graduate School of Public Health, University of Pittsburgh, Pittsburgh 13, Pa.

Isolation of pure sphingomyelin from human plasma on a preparative scale is described. Analyses of fatty acids by gas-liquid chromatography on two polar polyester columns indicate the presence of all even- and odd-carbon, saturated fatty acids from C₁₄ to C₂₅ and most of the corresponding monoenoic acids as well. Palmitic acid is the predominant component; seven of the acids constitute over 90% of the total fraction. A previously unassigned trace constituent of the fatty acid fraction has been tentatively identified as a C₂₄ dienoic acid on the basis of relative retention times, microhydrogenation, and reaction with mercuric acetate.

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