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Journal of Lipid Research, Vol. 4, 419-423, October 1963
Copyright © 1963 by Lipid Research, Inc.

Effect of heparin on disappearance of the cholesterol moiety of an injected cholesterol-C¹⁴-labeled, very low-density chyle lipoprotein fraction from the circulation of the rat

W. J. Lossow , S. S. Naidoo , and I. L. Chaikoff

Department of Physiology, University of California, Berkeley, California

A cholesterol-4-C¹⁴-labeled, very low-density, lipoprotein fraction of chyle was injected into heparin-treated and control rats. The disappearance of the C¹⁴ from the whole blood was followed at intervals up to 25 min after the injection. Heparin increased the rate at which the injected cholesterol-C¹⁴ left the circulation during the first 10 min. Determination of the distribution of isotope among ultracentrifugally separated S_f > 20, S_f 0-20, and high-density plasma lipoproteins revealed that, at 10 min after the administration of the cholesterol-labeled chyle preparation, a much greater proportion of the plasma lipid-C¹⁴ was present in the S_f 0-20 lipoproteins isolated from the heparin-treated than from the control rats. The in vitro mixing of the cholesterol-labeled, very low-density chyle lipoproteins with blood obtained from heparin-treated and control rats also resulted in recovery of a disproportionately high percentage of the isotope in the S_f 0-20 lipoproteins of the heparin-treated rats. Such a distribution of isotope among the plasma lipoproteins in heparin-treated rats is compatible with the scheme of Lindgren and co-workers for the action of clearing factor lipase in which chylomicrons are degraded through a series of lipoprotein complexes to lipoproteins of the S_f 0-20 class, the cholesterol and phospholipid components of the original chylomicrons becoming part of the final end products. A very rapid removal of any of the lipoproteins resulting from the heparin-induced lipolysis could account for the effect of heparin on the disappearance of the injected cholesterol-C¹⁴ of the chyle lipoproteins from the circulation.

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