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Journal of Lipid Research, Vol. 4, 444-447, October 1963
Copyright © 1963 by Lipid Research, Inc.

Biosynthesis of nervonic acid and its homologues from carboxyl-labeled oleic acid

Yasuo Kishimoto and Norman S. Radin

Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan

An emulsion of carboxyl-labeled potassium oleate was injected directly into the brains of a group of rats, and 24 hr later the rats were sacrificed. The brain sphingolipids were isolated, and from these, the individual normal unsaturated acids. The major acid, nervonic, was cleaved by ozonolysis and also by decarboxylation. Virtually no activity was found in the chain portion from the -end, while 74% of the activity was found in the carboxyl group. From these and other data, it would appear that very little oleate entered the cells that make the sphingolipid acids and that much of it was degraded to acetate. The labeled acetate thus formed was used to lengthen shorter acids (endogenous 18:1, 20:1, and 22:1). Apparently, there are pools of metabolically active unsaturated acids of various chain lengths, which can be incorporated into the sphingolipids or lengthened.

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