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Journal of Lipid Research, Vol. 4, 448-453, October 1963
Copyright © 1963 by Lipid Research, Inc.

In vivo conversion of labeled fatty acids to the sphingolipid fatty acids in rat brain

Amiya K. Hajra and Norman S. Radin

Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan

Emulsions of carboxyl-labeled palmitate, stearate, lignocerate, and dl-cerebronate were injected into the brains of weanling rats. After various time intervals, the rats were sacrificed, and the saturated fatty acids of the brain sphingolipids were isolated and decarboxylated. All four acids had been incorporated into the sphingolipids. The palmitate and stearate gave rise to radioactive lignocerate and cerebronate in which the carboxyl groups were relatively nonradioactive, indicating the latter acids are made by chain lengthening of the shorter acids. The lignocerate-C¹⁴ gave rise to labeled cerebronate in which the carboxyl group contained nearly all the activity, showing that the normal acid is converted fairly directly into the hydroxy acid. Some radioactivity was found also in the shorter acids, apparently by the intermediate degradation of lignocerate to acetate. The injected cerebronate-C¹⁴ underwent little or no conversion to normal acids.

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