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The Journal of Lipid Research, Vol. 40, 160-163, January 1999
Copyright © 1999 by Lipid Research, Inc.

Article on Methodology

A simple, nonenzymatic method for desialylating polysialylated ganglio-N-tetraose series gangliosides to produce GM1

Cara-Lynne Schengrunda and Pavol Kovácb
a Department of Biochemistry and Molecular Biology, The Pennsylvania State University College of Medicine, Hershey, PA 17033
b National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Building 8A, Bethesda, MD 20892

Correspondence to: Cara-Lynne Schengrund

Dowex-50W-H+ was used to catalyze the highly selective desialylation of polysialylated ganglio-N-tetraose series gangliosides to yield primarily GM1. High performance thin-layer chromatographic analysis of recovered lipid indicated that 60–70% of the recovered ganglioside was GM1. Identification of the major product as GM1 was confirmed by proton NMR spectra and lack of sialic acid release by Vibrio cholerae sialidase.—Schengrund, C-L., and P. Kovác. A simple, nonenzymatic method for desialylating polysialylated ganglio-N-tetraose series gangliosides to produce GM1. J. Lipid Res. 1999. 40: 160–163.

Supplementary key words: ganglioside GM1, Dowex-H+, sialic acid


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