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The Journal of Lipid Research, Vol. 40, 17-23, January 1999
Copyright © 1999 by Lipid Research, Inc.
The bile acid-inducible bai F gene from Eubacterium sp. strain VPI 12708 encodes a bile acid-coenzyme A hydrolase
Hua-Qing Yea,
Darrell H. Malloneea,
James E. Wellsa,
Ingemar Björkhemb, and
Phillip B. Hylemona
a Department of Microbiology/Immunology, Virginia Commonwealth University, Medical College of Virginia Campus, Richmond, VA 23298-0678
b Division of Clinical Chemistry, Karolinska Institutet, Huddinge Hospital, Huddinge, Sweden
Correspondence to:
Phillip B. Hylemon
The human intestinal Eubacterium sp. strain VPI 12708 has been shown to have a multistep biochemical pathway for bile acid 7 -dehydroxylation. A bile acid-inducible operon encoding 9 open reading frames has been cloned and sequenced from this organism. Several of the genes in this operon have been shown to catalyze specific reactions in the 7 -dehydroxylation pathway. The baiF gene from this operon was cloned, expressed in Escherichia coli, and found to encode a novel bile acid-coenzyme A (CoA) hydrolase. The subunit molecular mass of the purified bile acid-CoA hydrolase was calculated to be 47,466 daltons and the native enzyme had a relative molecular weight of 72,000. The K m and Vmax for cholyl-coenzyme A (CoA) hydrolysis was approximately 175 µM and 374 µmol/min per mg protein, respectively. The enzyme used cholyl-CoA, 3-dehydrocholyl-CoA, and chenodeoxycholyl-CoA as substrates. No hydrolytic activity was detected using acetyl-CoA, isovaleryl-CoA, palmitoyl-CoA, or phenylacetyl-CoA as substrates. Amino acid sequence database searches showed no significant similarity of bile acid-CoA hydrolase to other thioesterases, but significant amino acid sequence identity was found with Escherichia coli carnitine dehydratase. The characteristic thioesterase active site Gly-X-Ser-X-Gly motif was not found in the amino acid sequence of this enzyme.
Bile acid-CoA hydrolase from Eubacterium sp. strain VPI 12708 may represent a new family of thioesterases.Ye, H-Q., D. H. Mallonee, J. E. Wells, I. Björkem, and P. B. Hylemon. The bile acid-inducible baiF gene from Eubacterium sp. strain VPI 12708 encodes a bile acid-coenzyme A hydrolase. J. Lipid Res. 1999. 40: 1723.
Supplementary key words:
7 -dehydroxylation, secondary bile acid

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Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.
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