J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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Journal of Lipid Research, Vol. 40, 1867-1874, October 1999
Copyright © 1999 by Lipid Research, Inc.

Original Article

Blood compartmental metabolism of docosahexaenoic acid (DHA) in humans after ingestion of a single dose of [13C]DHA in phosphatidylcholine

Dominique Lemaitre-Delaunaya,b, Christiane Pachiaudic, Martine Lavillec, Jérome Pousind, Michael Armstrongb, and Michel Lagardea
a INSERM U352, Biochimie & Pharmacologie, INSA-Lyon, Villeurbanne
b Thallia Pharmaceuticals SA, Chemin de la Forestière, Ecully
c CRNHL & INSERM U449, Lyon
d Laboratoire de Modélisation Mathématique & Calcul Scientifique, UMR CNRS 5585, INSA-Lyon, France

Correspondence to: Michel Lagarde

The amount and distribution of [13C]docosahexaenoic acid (DHA) in plasma, platelet, and erythrocyte lipid classes were followed as a function of time (1 to 72 h) in young adults after ingestion of a single dose of [13C]DHA esterified in a phosphatidylcholine (PC), in using gas chromatography combustion;–isotope ratio mass spectrometry. [13C]DHA first appeared in plasma non-esterified fatty acids (NEFA) and triglycerides (TG), with a maximal appearance at 6 h and a further decline, then being delayed 3-fold compared to [13C]DHA ingested in triglycerides. Lysophosphatidylcholine (LPC) was also enriched in [13C]DHA, due mainly to earlier hepatic secretion, and plateaued at 6 h, whereas phosphatidylethanolamine (PE) and phosphatidylcholine (PC) containing [13C]DHA plateaued at 9 h. The labeling of erythrocyte and platelet phospholipids exhibited different kinetics, probably involving different metabolic pathways for [13C]DHA incorporation in cell membranes. Computation of the relative contribution of LPC and NEFA for delivery of [13C]DHA to blood cells showed that the supply to platelets occurred through NEFA. In contrast, [13C]DHA was carried by both LPC and NEFA to erythrocytes, which differs from what was previously been observed after intake of triglycerides labeled with [13C]DHA where LPC was the only source of [13C]DHA for erythrocytes.

We conclude that the lipid form of ingested DHA affects markedly its kinetics and partly its metabolic fate.—Lemaitre-Delaunay, D., C. Pachiaudi, M. Laville, J. Pousin, M. Armstrong, and M. Lagarde. Blood compartmental metabolism of docosahexaenoic acid (DHA) in humans after ingestion of a single dose of [13C]DHA in phosphatidylcholine. J. Lipid Res. 1999. 40: 1867;–1874.

Supplementary key words: docosahexaenoic acid, stable isotope, plasma, platelets, erythrocytes


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