HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Large, V. Articles by Arner, P. Search for Related Content PubMed PubMed Citation Articles by Large, V. Articles by Arner, P. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 40, 2059-2066, November 1999
Copyright © 1999 by Lipid Research, Inc.

Original Article

Decreased expression and function of adipocyte hormone-sensitive lipase in subcutaneous fat cells of obese subjects

Correspondence to: Peter Arner

Decreased lipolytic effect of catecholamines in adipose tissue has repeatedly been demonstrated in obesity and may be a cause of excess accumulation of body fat. However, the mechanisms behind this lipolysis defect are unclear. The role of hormone-sensitive lipase was examined using abdominal subcutaneous adipocytes from 34 obese drug-free and otherwise healthy males or females and 14 non-obese control subjects. The enzyme catalyzes the rate-limiting step of the lipolysis pathway. The maximum lipolytic capacity of fat cells was significantly decreased in obesity when measured using either a non-selective beta-adrenergic receptor agonist (isoprenaline) or a phosphodiesterase resistant cyclic AMP analogue (dibutyryl cyclic AMP). Likewise, enzyme activity, protein expression, and mRNA of hormone-sensitive lipase were significantly decreased in adipocytes of obese subjects. The findings were not influenced by age or gender.

The data suggest that a decreased expression of hormone-sensitive lipase in subcutaneous fat cells, which in turn causes decreased enzyme function and impaired lipolytic capacity of adipocytes, is present in obesity. Impaired expression of the hormone-sensitive lipase gene might at least in part explain the enzyme defect.—Large, V., S. Reynisdottir, D. Langin, K. Fredby, M. Klannemark, C. Holm, and P. Arner. Decreased expression and function of adipocyte hormone-sensitive lipase in subcutaneous fat cells of obese subjects. J. Lipid Res. 1999. 40: 2059;–2065.

Supplementary key words: mRNA, enzymes, lipolysis, catecholamines, obesity, lipase, adipose tissue, hormone sensitive lipase

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. W.E. Jocken, C. Roepstorff, G. H. Goossens, P. van der Baan, M. van Baak, W. H.M. Saris, B. Kiens, and E. E. Blaak Hormone-Sensitive Lipase Serine Phosphorylation and Glycerol Exchange Across Skeletal Muscle in Lean and Obese Subjects: Effect of {beta}-Adrenergic Stimulation Diabetes, July 1, 2008; 57(7): 1834 - 1841. [Abstract] [Full Text] [PDF]

				T. Agustsson, M. Ryden, J. Hoffstedt, V. van Harmelen, A. Dicker, J. Laurencikiene, B. Isaksson, J. Permert, and P. Arner Mechanism of Increased Lipolysis in Cancer Cachexia Cancer Res., June 1, 2007; 67(11): 5531 - 5537. [Abstract] [Full Text] [PDF]

				J. W. E. Jocken, D. Langin, E. Smit, W. H. M. Saris, C. Valle, G. B. Hul, C. Holm, P. Arner, and E. E. Blaak Adipose Triglyceride Lipase and Hormone-Sensitive Lipase Protein Expression Is Decreased in the Obese Insulin-Resistant State J. Clin. Endocrinol. Metab., June 1, 2007; 92(6): 2292 - 2299. [Abstract] [Full Text] [PDF]

				L. E. Johansson, J. Hoffstedt, H. Parikh, E. Carlsson, M. Wabitsch, A.-G. Bondeson, J. Hedenbro, H. Tornqvist, L. Groop, and M. Ridderstrale Variation in the Adiponutrin Gene Influences Its Expression and Associates With Obesity Diabetes, March 1, 2006; 55(3): 826 - 833. [Abstract] [Full Text] [PDF]

				T. Deng, S. Shan, P.-P. Li, Z.-F. Shen, X.-P. Lu, J. Cheng, and Z.-Q. Ning Peroxisome Proliferator-Activated Receptor-{gamma} Transcriptionally Up-Regulates Hormone-Sensitive Lipase via the Involvement of Specificity Protein-1 Endocrinology, February 1, 2006; 147(2): 875 - 884. [Abstract] [Full Text] [PDF]

				D. Langin, A. Dicker, G. Tavernier, J. Hoffstedt, A. Mairal, M. Ryden, E. Arner, A. Sicard, C. M. Jenkins, N. Viguerie, et al. Adipocyte Lipases and Defect of Lipolysis in Human Obesity Diabetes, November 1, 2005; 54(11): 3190 - 3197. [Abstract] [Full Text] [PDF]

				T. You, D. M. Berman, A. S. Ryan, and B. J. Nicklas Effects of Hypocaloric Diet and Exercise Training on Inflammation and Adipocyte Lipolysis in Obese Postmenopausal Women J. Clin. Endocrinol. Metab., April 1, 2004; 89(4): 1739 - 1746. [Abstract] [Full Text] [PDF]

				B. Mittendorfer, D. A. Fields, and S. Klein Excess body fat in men decreases plasma fatty acid availability and oxidation during endurance exercise Am J Physiol Endocrinol Metab, March 1, 2004; 286(3): E354 - E362. [Abstract] [Full Text]

				H. H. Zhang, S. C. Souza, K. V. Muliro, F. B. Kraemer, M. S. Obin, and A. S. Greenberg Lipase-selective Functional Domains of Perilipin A Differentially Regulate Constitutive and Protein Kinase A-stimulated Lipolysis J. Biol. Chem., December 19, 2003; 278(51): 51535 - 51542. [Abstract] [Full Text] [PDF]

				F. Diraison, V. Yankah, D. Letexier, E. Dusserre, P. Jones, and M. Beylot Differences in the regulation of adipose tissue and liver lipogenesis by carbohydrates in humans J. Lipid Res., April 1, 2003; 44(4): 846 - 853. [Abstract] [Full Text] [PDF]

				S. Lucas, G. Tavernier, C. Tiraby, A. Mairal, and D. Langin Expression of human hormone-sensitive lipase in white adipose tissue of transgenic mice increases lipase activity but does not enhance in vitro lipolysis J. Lipid Res., January 1, 2003; 44(1): 154 - 163. [Abstract] [Full Text] [PDF]

				F. B. Kraemer and W.-J. Shen Hormone-sensitive lipase: control of intracellular tri-(di-)acylglycerol and cholesteryl ester hydrolysis J. Lipid Res., October 1, 2002; 43(10): 1585 - 1594. [Abstract] [Full Text] [PDF]

				P. Lofgren, J. Hoffstedt, M. Ryden, A. Thorne, C. Holm, H. Wahrenberg, and P. Arner Major Gender Differences in the Lipolytic Capacity of Abdominal Subcutaneous Fat Cells in Obesity Observed before and after Long-Term Weight Reduction J. Clin. Endocrinol. Metab., February 1, 2002; 87(2): 764 - 771. [Abstract] [Full Text] [PDF]

				F. Smih, P. Rouet, S. Lucas, A. Mairal, C. Sengenes, M. Lafontan, S. Vaulont, M. Casado, and D. Langin Transcriptional Regulation of Adipocyte Hormone-Sensitive Lipase by Glucose Diabetes, February 1, 2002; 51(2): 293 - 300. [Abstract] [Full Text] [PDF]

				F. Diraison, E. Dusserre, H. Vidal, M. Sothier, and M. Beylot Increased hepatic lipogenesis but decreased expression of lipogenic gene in adipose tissue in human obesity Am J Physiol Endocrinol Metab, January 1, 2002; 282(1): E46 - E51. [Abstract] [Full Text] [PDF]

				J. Hoffstedt, P. Arner, M. Schalling, N. L. Pedersen, S. Sengul, S. Ahlberg, A. Iliadou, and C. Lavebratt A Common Hormone-Sensitive Lipase i6 Gene Polymorphism Is Associated With Decreased Human Adipocyte Lipolytic Function Diabetes, October 1, 2001; 50(10): 2410 - 2413. [Abstract] [Full Text]

				S. Meijssen, M. C. Cabezas, C. G. M. Ballieux, R. J. Derksen, S. Bilecen, and D. W. Erkelens Insulin Mediated Inhibition of Hormone Sensitive Lipase Activity in Vivo in Relation to Endogenous Catecholamines in Healthy Subjects J. Clin. Endocrinol. Metab., September 1, 2001; 86(9): 4193 - 4197. [Abstract] [Full Text] [PDF]

				M. Elizalde, M. Rydén, V. van Harmelen, P. Eneroth, H. Gyllenhammar, C. Holm, S. Ramel, A. Ölund, P. Arner, and K. Andersson Expression of nitric oxide synthases in subcutaneous adipose tissue of nonobese and obese humans J. Lipid Res., August 1, 2000; 41(8): 1244 - 1251. [Abstract] [Full Text]