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Journal of Lipid Research, Vol. 40, 2169-2176, December 1999
Copyright © 1999 by Lipid Research, Inc.

Original Article

Isolation and characterization of monoacetyldiglycerides from bovine udder

Jin-Kyung Limba, Young Hwan Kimb, So-Yeop Hana, and Gil-Ja Jhona
a Department of Chemistry, Division of Molecular Life Science and Center for Cell Signaling Research, Ewha Womans University, Seoul 120-750, Korea
b Mass Spectrometry Group, Korea Basic Science Institute, Taejon 305-600, Korea

Correspondence to: Gil-Ja Jhon

Monoacetyldiglycerides (MADGs) were isolated from an animal tissue, bovine udder, by solvent extraction and silica gel column chromatography. Monoacetyldiglyceride structures were identified using a variety of 1-D and 2-D NMR techniques and collision-induced dissociation (CID) tandem mass spectrometry coupled with fast atom bombardment. CID of sodium-adducted molecular ions ([M+ Na]+) generated numerous types of product ions providing information on the double bond position of fatty acyl groups as well as fatty acid compositions. Structural analysis led to the classification of the MADGs isolated from bovine udder as 1-palmitoyl-2-lauroyl-3-acetyl-rac-glycerol, 1,2-dimyristoyl-3-acetyl-rac-glycerol, 1-palmitoyl-2-myristoyl-3-acetyl-rac-glycerol, 1-oleoyl-2-myristoyl-3-acetyl-rac-glycerol, 1-palmitoyl-2-palmitoleoyl-3-acetyl-rac-glycerol, 1,2-dipalmitoyl-3-acetyl-rac-glycerol, 1-linoleoyl-2-palmitoyl-3-acetyl-rac-glycerol, 1-oleoyl-2-palmitoleoyl-3-acetyl-rac-glycerol, 1-oleoyl-2-palmitoyl-3-acetyl-rac-glycerol, 1-stearoyl-2-palmitoyl-3-acetyl-rac-glycerol, 1-oleoyl-2-linoleoyl-3-acetyl-rac-glycerol, 1,2-dioleoyl-3-acetyl-rac-glycerol, and 1-stearoyl-2-oleoyl-3-acetyl-rac-glycerol.—Limb, J-K., Y. H. Kim, S-Y. Han, and G-J. Jhon. Isolation and characterization of monoacetyldiglycerides from bovine udder. J. Lipid Res. 1999. 40: 2169;–2176.

Supplementary key words: monoacetyldiglycerides, bovine udder, 1-D and 2-D NMR, fast atom bombardment, tandem mass spectrometry


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