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Correspondence to:
George H. Rothblat
In addition to its effect on high density lipoprotein (HDL) cholesteryl ester (CE) uptake, scavenger receptor BI (SR-BI) was recently reported to stimulate free cholesterol (FC) flux from Chinese hamster ovary (CHO) cells stably expressing mouse SR-BI, a novel function of SR-BI that may play a role in cholesterol removal from the vessel wall where the receptor can be found. It is possible that SR-BI stimulates flux simply by tethering acceptor HDL particles in close apposition to the cell surface thereby facilitating the movement of cholesterol between the plasma membrane and HDL. To test this, we used transiently transfected cells and compared the closely related class B scavenger receptors mouse SR-BI and rat CD36 for their ability to stimulate cholesterol efflux as both receptors bind HDL with high affinity. The results showed that, although acceptor binding to SR-BI may contribute to efflux to a modest extent, the major stimulation of FC efflux occurs independently of acceptor binding to cell surface receptors. Instead our data indicate that SR-BI mediates alterations to membrane FC domains which provoke enhanced bidirectional FC flux between cells and extracellular acceptors.—de la Llera-Moya, M., G. H. Rothblat, M. A. Connelly, G. Kellner-Weibel, S. W. Sakr, M. C. Phillips, and D. L. Williams. Scavenger receptor BI (SR-BI) mediates free cholesterol flux independently of HDL tethering to the cell surface. J. Lipid Res. 1999. 40: 575–580.
Supplementary key words:
cholesterol, SR-BI, CD36, scavenger receptor, efflux, HDL, reverse cholesterol transport
Copyright © 1999 by Lipid Research, Inc.
Rapid Communication
Scavenger receptor BI (SR-BI) mediates free cholesterol flux independently of HDL tethering to the cell surface
Margarita de la Llera-Moyaa,
George H. Rothblata,
Margery A. Connellyb,
Ginny Kellner-Weibela,
Sana W. Sakra,
Michael C. Phillipsa, and
David L. Williamsb
a Department of Biochemistry, MCP 
Hahnemann School of Medicine, Philadelphia PA, 19129
b Department of Pharmacological Sciences, SUNY at Stony Brook, University Medical Center, Stony Brook, NY 11794
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