HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Atshaves, B. P. Articles by Schroeder, F. Search for Related Content PubMed PubMed Citation Articles by Atshaves, B. P. Articles by Schroeder, F. Social Bookmarking                      What's this?

The Journal of Lipid Research, Vol. 40, 610-622, April 1999
Copyright © 1999 by Lipid Research, Inc.

Original Article

Expression and intracellular processing of the 58 kDa sterol carrier protein-2/3-oxoacyl-CoA thiolase in transfected mouse L-cell fibroblasts

Correspondence to: Friedhelm Schroeder

Although the sterol carrier protein 2 (SCP-2) gene encodes for two proteins, almost nothing is known of the function and potential processing of the larger transcript corresponding to the 58 kDa sterol carrier protein-2/3-oxoacyl-CoA thiolase (SCP-x), in intact cells. L-cell fibroblasts transfected with cDNA encoding for the 58 kDa SCP-x protein had a 4.5-fold increase in SCP-x mRNA transcript levels. Western blot analysis showed SCP-x protein expression reached 0.011% of total protein, representing a 4.1-fold increase over basal levels. Surprisingly, the 13.2 kDa SCP-2 protein also increased 2-fold in the transfected cells. This was consistent with part of the 58 kDa SCP-x being proteolytically processed to 13.2 kDa SCP-2 as there was no evidence of an mRNA transcript corresponding to a 13.2/15.2 kDa gene product in the transfected L-cell clones. Confocal immunofluorescence microscopy of transfected L-cells showed that SCP-x/SCP-2 co-localized in highest concentration with catalase in peroxisomes, but significant amounts appeared extra-peroxisomal. Overexpression of SCP-x significantly altered cholesterol uptake and metabolism. Uptake of exogenous [³H]cholesterol and total cholesterol mass were increased 1.9- and 1.4-fold, respectively, in SCP-x expressors. Although cholesterol ester mass was unaltered, incorporation of exogenous [³H]cholesterol and [³H]oleic acid into cholesteryl esters increased 2.3- and 2.5-fold, respectively.

These results from intact cells suggest the 13.2 kDa SCP-2 can arise from the larger SCP-2 gene product and indicate a role for the 58 kDa SCP-x protein in cholesterol uptake and intracellular cycling.—Atshaves, B. P., A. D. Petrescu, O. Starodub, J. B. Roths, A. B. Kier, and F. Schroeder. Expression and intracellular processing of the 58 kDa sterol carrier protein-2/3-oxoacyl-CoA thiolase in transfected mouse L-cell fibroblasts. J. Lipid Res. 1999. 40: 610 – 622.

Supplementary key words: sterol carrier protein-2, sterol carrier protein-2/3-oxoacyl-CoA thiolase, L-cell fibroblast, cholesterol trafficking

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				G. G. Martin, B. P. Atshaves, H. Huang, A. L. McIntosh, B. J. Williams, P.-J. Pai, D. H. Russell, A. B. Kier, and F. Schroeder Hepatic phenotype of liver fatty acid binding protein gene-ablated mice Am J Physiol Gastrointest Liver Physiol, December 1, 2009; 297(6): G1053 - G1065. [Abstract] [Full Text] [PDF]

				H. A. Hostetler, A. L. McIntosh, B. P. Atshaves, S. M. Storey, H. R. Payne, A. B. Kier, and F. Schroeder L-FABP directly interacts with PPAR{alpha} in cultured primary hepatocytes J. Lipid Res., August 1, 2009; 50(8): 1663 - 1675. [Abstract] [Full Text] [PDF]

				B. P. Atshaves, A. L. McIntosh, G. G. Martin, D. Landrock, H. R. Payne, S. Bhuvanendran, K. K. Landrock, O. I. Lyuksyutova, J. D. Johnson, R. D. Macfarlane, et al. Overexpression of sterol carrier protein-2 differentially alters hepatic cholesterol accumulation in cholesterol-fed mice J. Lipid Res., July 1, 2009; 50(7): 1429 - 1447. [Abstract] [Full Text] [PDF]

				K. T. Burke, P. L. Colvin, L. Myatt, G. A. Graf, F. Schroeder, and L. A. Woollett Transport of maternal cholesterol to the fetus is affected by maternal plasma cholesterol concentrations in the Golden Syrian hamster J. Lipid Res., June 1, 2009; 50(6): 1146 - 1155. [Abstract] [Full Text] [PDF]

				J. T. Mackie, B. P. Atshaves, H. R. Payne, A. L. McIntosh, F. Schroeder, and A. B. Kier Phytol-induced Hepatotoxicity in Mice Toxicol Pathol, February 1, 2009; 37(2): 201 - 208. [Abstract] [Full Text] [PDF]

				G. G. Martin, B. P. Atshaves, A. L. McIntosh, J. T. Mackie, A. B. Kier, and F. Schroeder Liver Fatty Acid-Binding Protein Gene-Ablated Female Mice Exhibit Increased Age-Dependent Obesity J. Nutr., October 1, 2008; 138(10): 1859 - 1865. [Abstract] [Full Text] [PDF]

				B. P. Atshaves, A. L. McIntosh, H. R. Payne, A. M. Gallegos, K. Landrock, N. Maeda, A. B. Kier, and F. Schroeder SCP-2/SCP-x gene ablation alters lipid raft domains in primary cultured mouse hepatocytes J. Lipid Res., October 1, 2007; 48(10): 2193 - 2211. [Abstract] [Full Text] [PDF]

				B. P. Atshaves, A. L. McIntosh, D. Landrock, H. R. Payne, J. T. Mackie, N. Maeda, J. Ball, F. Schroeder, and A. B. Kier Effect of SCP-x gene ablation on branched-chain fatty acid metabolism Am J Physiol Gastrointest Liver Physiol, March 1, 2007; 292(3): G939 - G951. [Abstract] [Full Text] [PDF]

				T. Kriska, V. V. Levchenko, W. Korytowski, B. P. Atshaves, F. Schroeder, and A. W. Girotti Intracellular Dissemination of Peroxidative Stress: INTERNALIZATION, TRANSPORT, AND LETHAL TARGETING OF A CHOLESTEROL HYDROPEROXIDE SPECIES BY STEROL CARRIER PROTEIN-2-OVEREXPRESSING HEPATOMA CELLS J. Biol. Chem., August 18, 2006; 281(33): 23643 - 23651. [Abstract] [Full Text] [PDF]

				G. G. Martin, B. P. Atshaves, A. L. McIntosh, J. T. Mackie, A. B. Kier, and F. Schroeder Liver fatty acid binding protein gene ablation potentiates hepatic cholesterol accumulation in cholesterol-fed female mice Am J Physiol Gastrointest Liver Physiol, January 1, 2006; 290(1): G36 - G48. [Abstract] [Full Text] [PDF]

				B. P. Atshaves, A. L. McIntosh, H. R. Payne, J. Mackie, A. B. Kier, and F. Schroeder Effect of branched-chain fatty acid on lipid dynamics in mice lacking liver fatty acid binding protein gene Am J Physiol Cell Physiol, March 1, 2005; 288(3): C543 - C558. [Abstract] [Full Text] [PDF]

				Q. Lan and R. J. Massey Subcellular localization of the mosquito sterol carrier protein-2 and sterol carrier protein-x J. Lipid Res., August 1, 2004; 45(8): 1468 - 1474. [Abstract] [Full Text] [PDF]

				B. P. Atshaves, A. M. McIntosh, O. I. Lyuksyutova, W. Zipfel, W. W. Webb, and F. Schroeder Liver Fatty Acid-binding Protein Gene Ablation Inhibits Branched-chain Fatty Acid Metabolism in Cultured Primary Hepatocytes J. Biol. Chem., July 23, 2004; 279(30): 30954 - 30965. [Abstract] [Full Text] [PDF]

				B. P. Atshaves, H. R. Payne, A. L. McIntosh, S. E. Tichy, D. Russell, A. B. Kier, and F. Schroeder Sexually dimorphic metabolism of branched-chain lipids in C57BL/6J mice J. Lipid Res., May 1, 2004; 45(5): 812 - 830. [Abstract] [Full Text] [PDF]

				T. B. Dansen, G. J. P. L. Kops, S. Denis, N. Jelluma, R. J. A. Wanders, J. L. Bos, B. M. T. Burgering, and K. W. A. Wirtz Regulation of sterol carrier protein gene expression by the Forkhead transcription factor FOXO3a J. Lipid Res., January 1, 2004; 45(1): 81 - 88. [Abstract] [Full Text] [PDF]

				B. P. Atshaves, S. M. Storey, and F. Schroeder Sterol carrier protein-2/sterol carrier protein-x expression differentially alters fatty acid metabolism in L cell fibroblasts J. Lipid Res., September 1, 2003; 44(9): 1751 - 1762. [Abstract] [Full Text] [PDF]

				G. G. Martin, H. Danneberg, L. S. Kumar, B. P. Atshaves, E. Erol, M. Bader, F. Schroeder, and B. Binas Decreased Liver Fatty Acid Binding Capacity and Altered Liver Lipid Distribution in Mice Lacking the Liver Fatty Acid-binding Protein Gene J. Biol. Chem., June 6, 2003; 278(24): 21429 - 21438. [Abstract] [Full Text] [PDF]

				H. Chao, M. Zhou, A. McIntosh, F. Schroeder, and A. B. Kier ACBP and cholesterol differentially alter fatty acyl CoA utilization by microsomal ACAT J. Lipid Res., January 1, 2003; 44(1): 72 - 83. [Abstract] [Full Text] [PDF]

				B. P. Atshaves, S. M. Storey, A. Petrescu, C. C. Greenberg, O. I. Lyuksyutova, R. Smith III, and F. Schroeder Expression of fatty acid binding proteins inhibits lipid accumulation and alters toxicity in L cell fibroblasts Am J Physiol Cell Physiol, September 1, 2002; 283(3): C688 - C703. [Abstract] [Full Text] [PDF]

				F. Schroeder, A. M. Gallegos, B. P. Atshaves, S. M. Storey, A. L. McIntosh, A. D. Petrescu, H. Huang, O. Starodub, H. Chao, H. Yang, et al. Recent Advances in Membrane Microdomains: Rafts, Caveolae, and Intracellular Cholesterol Trafficking Exp Biol Med, November 1, 2001; 226(10): 873 - 890. [Abstract] [Full Text] [PDF]

				O. Starodub, C. A. Jolly, B. P. Atshaves, J. B. Roths, E. J. Murphy, A. B. Kier, and F. Schroeder Sterol carrier protein-2 localization in endoplasmic reticulum and role in phospholipid formation Am J Physiol Cell Physiol, October 1, 2000; 279(4): C1259 - C1269. [Abstract] [Full Text] [PDF]

				E. J. Murphy, T. Stiles, and F. Schroeder Sterol carrier protein-2 expression alters phospholipid content and fatty acyl composition in L-cell fibroblasts J. Lipid Res., May 1, 2000; 41(5): 788 - 796. [Abstract] [Full Text]

				N. Stolowich, A. Frolov, A. D. Petrescu, A. I. Scott, J. T. Billheimer, and F. Schroeder Holo-sterol Carrier Protein-2. 13C NMR INVESTIGATION OF CHOLESTEROL AND FATTY ACID BINDING SITES J. Biol. Chem., December 10, 1999; 274(50): 35425 - 35433. [Abstract] [Full Text] [PDF]

				F. Schroeder, A. Frolov, O. Starodub, B. B. Atshaves, W. Russell, A. Petrescu, H. Huang, A. M. Gallegos, A. McIntosh, D. Tahotna, et al. Pro-sterol Carrier Protein-2. ROLE OF THE N-TERMINAL PRESEQUENCE IN STRUCTURE, FUNCTION, AND PEROXISOMAL TARGETING J. Biol. Chem., August 11, 2000; 275(33): 25547 - 25555. [Abstract] [Full Text] [PDF]

				B. P. Atshaves, O. Starodub, A. McIntosh, A. Petrescu, J. B. Roths, A. B. Kier, and F. Schroeder Sterol Carrier Protein-2 Alters High Density Lipoprotein-mediated Cholesterol Efflux J. Biol. Chem., November 17, 2000; 275(47): 36852 - 36861. [Abstract] [Full Text] [PDF]

				B. P. Atshaves, S. M. Storey, A. L. McIntosh, A. D. Petrescu, O. I. Lyuksyutova, A. S. Greenberg, and F. Schroeder Sterol Carrier Protein-2 Expression Modulates Protein and Lipid Composition of Lipid Droplets J. Biol. Chem., June 29, 2001; 276(27): 25324 - 25335. [Abstract] [Full Text] [PDF]

				M. Hao, S. X. Lin, O. J. Karylowski, D. Wustner, T. E. McGraw, and F. R. Maxfield Vesicular and Non-vesicular Sterol Transport in Living Cells. THE ENDOCYTIC RECYCLING COMPARTMENT IS A MAJOR STEROL STORAGE ORGANELLE J. Biol. Chem., January 4, 2002; 277(1): 609 - 617. [Abstract] [Full Text] [PDF]