J. Lipid Res. Please sign the JLR Guestbook
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Maggio, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maggio, B.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
The Journal of Lipid Research, Vol. 40, 930-939, May 1999
Copyright © 1999 by Lipid Research, Inc.

Original Article

Modulation of phospholipase A2 by electrostatic fields and dipole potential of glycosphingolipids in monolayers

Bruno Maggioa
a Departamento de Quimica Biológica-CIQUIBIC, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Agencia Postal 4, CC 61, 5000 Córdoba, Argentina

Phospholipase A2 activity against mixed monolayers of dilauroylphosphatidic acid or dilauroylphosphatidylcholine with glycosphingolipids can be reversibly modulated by external constant electrostatic fields. The changes of enzymatic activity are correlated to the depolarization or hyperpolarization of the film caused by specific dipolar properties of glycosphingolipids. Hyperpolarizing fields enhance the enzymatic activity against pure dilauroylphosphatidic acid while depolarizing fields induce a decrease of activity. Compared to the pure substrate, the interface of mixed films containing neutral glycosphingolipids or gangliosides is already partially depolarized and the magnitude of activation induced by an external hyperpolarizing field is decreased; conversely, depolarizing fields cause an increased inhibition of activity. Differing from gangliosides, sulfatides bring about a hyperpolarization of the mixed lipid monolayer and external hyperpolarizing or depolarizing fields cause enhanced activation and reduced inhibition, respectively. The effects of glycosphingolipids depend on their relative proportion in the monolayer. Results were similar with dilauroylphosphosphatidylcholine but the field effects were less than half of those found with dilauroylphosphatidic acid.

Our work shows that the activity of phospholipase A2 in addition to responding reversibly to external electrostatic fields, is directly modulated by the polarity and magnitude of the lipid polar head group dipole moments.—Maggio, B. Modulation of phospholipase A2 by electrostatic fields and dipole potential of glycosphingolipids in monolayers. J. Lipid Res. 1999. 40: 930–939.

Supplementary key words: gangliosides, sulfatides, cerebrosides, enzyme activity in monolayers, surface potential


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Wang, P. S. Bose, and F. J. Sigworth
Using cryo-EM to measure the dipole potential of a lipid membrane
PNAS, December 5, 2006; 103(49): 18528 - 18533.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
T. Starke-Peterkovic, N. Turner, M. F. Vitha, M. P. Waller, D. E. Hibbs, and R. J. Clarke
Cholesterol Effect on the Dipole Potential of Lipid Membranes
Biophys. J., June 1, 2006; 90(11): 4060 - 4070.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
S. Hartel, M. L. Fanani, and B. Maggio
Shape Transitions and Lattice Structuring of Ceramide-Enriched Domains Generated by Sphingomyelinase in Lipid Monolayers
Biophys. J., January 1, 2005; 88(1): 287 - 304.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
H. L. Brockman, M. M. Momsen, R. E. Brown, L. He, J. Chun, H.-S. Byun, and R. Bittman
The 4,5-Double Bond of Ceramide Regulates Its Dipole Potential, Elastic Properties, and Packing Behavior
Biophys. J., September 1, 2004; 87(3): 1722 - 1731.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M. L. Fanani, S. Hartel, R. G. Oliveira, and B. Maggio
Bidirectional Control of Sphingomyelinase Activity and Surface Topography in Lipid Monolayers
Biophys. J., December 1, 2002; 83(6): 3416 - 3424.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Asawakarn, J. Cladera, and P. O'Shea
Effects of the Membrane Dipole Potential on the Interaction of Saquinavir with Phospholipid Membranes and Plasma Membrane Receptors of Caco-2 Cells
J. Biol. Chem., October 12, 2001; 276(42): 38457 - 38463.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.