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The Journal of Lipid Research, Vol. 40, 1007-1016, June 1999
Copyright © 1999 by Lipid Research, Inc.

Original Article

Protein-mediated palmitate uptake and expression of fatty acid transport proteins in heart giant vesicles

Joost J. F. P. Luikena, Lorraine P. Turcotteb, and Arend Bonena
a Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1
b Department of Exercise Sciences, University of Southern California, Los Angeles, CA 90089

Correspondence to: Arend Bonen

Giant sarcolemmal vesicles were isolated from rat heart and hindlimb muscles for a) characterization of long-chain fatty acid transport in the absence of metabolism and b) comparison of fatty acid transport protein expression with fatty acid transport. Giant vesicles contained cytosolic fatty acid binding protein. Palmitate uptake was completely divorced from its metabolism. All palmitate taken up was recovered in the intravesicular cytosol as unesterified FA. Palmitate uptake by heart vesicles exhibited a K m of 9.7 nM, similar to that of muscle (K m = 9.7 nM). Vmax (2.7 pmol/mg protein/s) in heart was 8-fold higher than in muscle (0.34 pmol/mg protein/s). Palmitate uptake was inhibited in heart (55–80%) and muscle (31–50%) by trypsin, phloretin, sulfo-N-succinimidyloleate (SSO), or a polyclonal antiserum against the 40 kDa plasma membrane fatty acid binding protein (FABPpm). Palmitate uptake by heart and by red and white muscle vesicles correlated well with the expression of fatty acid translocase (FAT/CD36) and fatty acid binding protein FABPpm, which may act in concert. The expression of fatty acid transport protein (FATP), was 10-fold lower in heart vesicles than in white muscle vesicles.

It is concluded that long-chain fatty acid uptake by heart and muscle vesicles is largely protein-mediated, involving FAT/CD36 and FABPpm. The role of FATP in muscle and heart remains uncertain.—Luiken, J. J. F. P., L. P. Turcotte, and A. Bonen. Protein-mediated palmitate uptake and expression of fatty acid transport proteins in heart giant vesicles. J. Lipid Res. 1999. 40: 1007–1016.

Supplementary key words: FAT/CD36, FABPpm, FATP, uptake kinetics, muscle


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