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The Journal of Lipid Research, Vol. 40, 1148-1154, June 1999
Copyright © 1999 by Lipid Research, Inc.

Methods

Highly simplified method for gas-liquid chromatographic quantitation of bile acids and sterols in human stool

Ashok Kumar Battaa, Gerald Salena,b, Keshav R. Rapolea, Manju Battab, Priti Battac, David Albertsd, and David Earnestd
a Department of Medicine, Gastroenterology Division, University of Medicine and Dentistry-New Jersey Medical School, Newark, NJ
b Department of Veterans Affairs Medical Center, East Orange, NJ
c Swarthmore College, Swarthmore, PA
d University of Arizona Medical Center, Tucson, AZ

Correspondence to: Ashok Kumar Batta

A simple method for the gas–liquid chromatographic quantitation of human fecal bile acids and sterols is described where bile acids are subjected to n-butyl ester derivatization, without prior isolation from the stool, followed by trimethylsilylation of the sterols and bile acids. Under these conditions, bile acid derivatives are well resolved from each other and from the trimethylsilyl ether derivatives of fecal sterols and no overlap occurs. The method was shown to be highly reproducible and recoveries were similar to those obtained with other methods used for fecal bile acid analysis. Application of the method for bile acid and sterol analysis in human stool is described.—Batta, A. K ., G. Salen, K . R. Rapole, M. Batta, P. Batta, D. Alberts, and D. Earnest. Highly simplified method for gas–liquid chromatographic quantitation of bile acids and sterols in human stool. J. Lipid Res. 1999. 40: 1148–1154.

Supplementary key words: bile acids, bile acid n-butyl ester-trimethylsilyl ethers, capillary gas–liquid chromatography, human fecal bile acids, fecal sterols, fatty acids


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