HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Alcouffe, J. Articles by Benoist, H. Search for Related Content PubMed PubMed Citation Articles by Alcouffe, J. Articles by Benoist, H. Social Bookmarking                      What's this?

The Journal of Lipid Research, Vol. 40, 1200-1210, July 1999
Copyright © 1999 by Lipid Research, Inc.

Original Article

Oxidized low density lipoproteins induce apoptosis in PHA-activated peripheral blood mononuclear cells and in the Jurkat T-cell line

Correspondence to: Hervé Benoist

Oxidized low density lipoproteins (oxLDLs) and activated T lymphocytes are present in early atherosclerotic plaques. It has been shown that oxLDLs are cytotoxic to cultured vascular cells but their possible toxic action on T lymphocytes has not been described. Peripheral blood lymphocytes from healthy individuals were stimulated in vitro with the polyclonal activator phytohemagglutinin and treated with various doses of native and mildly oxidized LDLs. Low doses of oxLDLs inhibited cell growth and DNA synthesis after 48 h culture and at 200 µg apoB/ml we observed a loss of cell viability. Dead cells did not exhibit significant increase of alteration of membrane integrity (i.e., necrosis) but showed chromatin fragmentation evaluated by DNA staining with 4',6-diamidino-2-phenylindole and propidium iodide. This fragmentation increased with TBARS and hydroperoxide levels. The expression of early apoptosis marker Apo2.7 rose among the CD3⁺ T-cell population. In addition, morphological analysis showed apoptotic features (cell shrinking, nucleus condensation, and fragmentation). Study of phosphatidylserine expression using Annexin V confirmed that oxLDLs induced apoptosis in activated lymphocytes. In the Jurkat T-cell line cultured with oxLDLs, apoptotic morphological changes (condensation and nucleus fragmentation) were observed and they were accompanied by DNA fragmentation visualized by propidium iodide staining and electrophoresis showing apoptotic ladder.

These results demonstrate that mildly oxidized LDLs induce apoptosis in a part of activated and proliferating T cells. T-lymphocyte apoptosis induction in atherosclerotic lesions might contribute to the development of an unappropriate local T cell response.—Alcouffe, J., S. Caspar-Bauguil, V. Garcia, R. Salvayre, M. Thomsen, and H. Benoist. Oxidized low density lipoproteins induce apoptosis in PHA-activated peripheral blood mononuclear cells and in the Jurkat T-cell line. J. Lipid Res. 1999. 40: 1200;–1210.

Supplementary key words: activated T lymphocyte, atherosclerosis, proliferation, cell death

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				N. R. Madamanchi and M. S. Runge Mitochondrial Dysfunction in Atherosclerosis Circ. Res., March 2, 2007; 100(4): 460 - 473. [Abstract] [Full Text] [PDF]

				T. Shimaoka, T. Nakayama, K. Hieshima, N. Kume, N. Fukumoto, M. Minami, K. Hayashida, T. Kita, O. Yoshie, and S. Yonehara Chemokines Generally Exhibit Scavenger Receptor Activity through Their Receptor-binding Domain J. Biol. Chem., June 25, 2004; 279(26): 26807 - 26810. [Abstract] [Full Text] [PDF]

				S. J. Hodge, G. L. Hodge, P. N. Reynolds, R. Scicchitano, and M. Holmes Increased production of TGF-{beta} and apoptosis of T lymphocytes isolated from peripheral blood in COPD Am J Physiol Lung Cell Mol Physiol, August 1, 2003; 285(2): L492 - L499. [Abstract] [Full Text] [PDF]

				R. Asmis and J. G. Begley Oxidized LDL Promotes Peroxide-Mediated Mitochondrial Dysfunction and Cell Death in Human Macrophages: A Caspase-3-Independent Pathway Circ. Res., January 10, 2003; 92 (1): e20 - e29. [Abstract] [Full Text] [PDF]