HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Spady, D. K. Articles by Hobbs, H. H. Search for Related Content PubMed PubMed Citation Articles by Spady, D. K. Articles by Hobbs, H. H. Social Bookmarking                      What's this?

The Journal of Lipid Research, Vol. 40, 1384-1394, August 1999
Copyright © 1999 by Lipid Research, Inc.

Original Article

Polyunsaturated fatty acids up-regulate hepatic scavenger receptor B1 (SR-BI) expression and HDL cholesteryl ester uptake in the hamster

Correspondence to: David K. Spady

Diets rich in polyunsaturated fatty acids lower plasma HDL cholesterol concentrations when compared to diets rich in saturated fatty acids. We investigated the mechanistic basis for this effect in the hamster and sought to determine whether reduced plasma HDL cholesterol concentrations resulting from a high polyunsaturated fat diet are associated with a decrease in reverse cholesterol transport. Animals were fed semisynthetic diets enriched with polyunsaturated or saturated fatty acids for 6 weeks. We then determined the effect of these diets on the following parameters: 1) hepatic scavenger receptor B1 (SR-BI) mRNA and protein levels, 2) the rate of hepatic HDL cholesteryl ester uptake, and 3) the rate of cholesterol acquisition by the extrahepatic tissues (from de novo synthesis, LDL and HDL) as a measure of the rate of reverse cholesterol transport. Compared to saturated fatty acids, dietary polyunsaturated fatty acids up-regulated hepatic SR-BI expression by ~50% and increased HDL cholesteryl ester transport to the liver; as a consequence, plasma HDL cholesteryl ester concentrations were reduced. Although dietary polyunsaturated fatty acids increased hepatic HDL cholesteryl ester uptake and lowered plasma HDL cholesterol concentrations, there was no change in the cholesterol content or in the rate of cholesterol acquisition (via de novo synthesis and lipoprotein uptake) by the extrahepatic tissues.

These studies indicate that substitution of polyunsaturated for saturated fatty acids in the diet increases SR-BI expression and lowers plasma HDL cholesteryl ester concentrations but does not affect reverse cholesterol transport.—Spady, D. K., D. M. Kearney, and H. H. Hobbs. Polyunsaturated fatty acids up-regulate hepatic scavenger receptor B1 (SR-BI) expression and HDL cholesteryl ester uptake in the hamster. J. Lipid Res. 1999. 40: 1384;–1394.

Supplementary key words: HDL, lipoproteins, cholesterol, cholesteryl esters, liver, reverse cholesterol transport

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. West, E. Greason, A. Kolmakova, A. Jahangiri, B. Asztalos, T. I. Pollin, and A. Rodriguez Scavenger Receptor Class B Type I Protein as an Independent Predictor of High-Density Lipoprotein Cholesterol Levels in Subjects with Hyperalphalipoproteinemia J. Clin. Endocrinol. Metab., April 1, 2009; 94(4): 1451 - 1457. [Abstract] [Full Text] [PDF]

				A. D. Mooradian, M. J. Haas, and N. C. W. Wong The Effect of Select Nutrients on Serum High-Density Lipoprotein Cholesterol and Apolipoprotein A-I Levels Endocr. Rev., February 1, 2006; 27(1): 2 - 16. [Abstract] [Full Text] [PDF]

				F. Maingrette and G. Renier Linoleic Acid Increases Lectin-Like Oxidized LDL Receptor-1 (LOX-1) Expression in Human Aortic Endothelial Cells Diabetes, May 1, 2005; 54(5): 1506 - 1513. [Abstract] [Full Text] [PDF]

				S. E. Dorfman, S. Wang, S. Vega-Lopez, M. Jauhiainen, and A. H. Lichtenstein Dietary Fatty Acids and Cholesterol Differentially Modulate HDL Cholesterol Metabolism in Golden-Syrian Hamsters J. Nutr., March 1, 2005; 135(3): 492 - 498. [Abstract] [Full Text] [PDF]

				B. L. Trigatti, M. Krieger, and A. Rigotti Influence of the HDL Receptor SR-BI on Lipoprotein Metabolism and Atherosclerosis Arterioscler. Thromb. Vasc. Biol., October 1, 2003; 23(10): 1732 - 1738. [Abstract] [Full Text] [PDF]

				S. V. Gupta, N. Yamada, T. V. Fungwe, and P. Khosla Replacing 40% of Dietary Animal Fat with Vegetable Oil Is Associated with Lower HDL Cholesterol and Higher Cholesterol Ester Transfer Protein in Cynomolgus Monkeys Fed Sufficient Linoleic Acid J. Nutr., August 1, 2003; 133(8): 2600 - 2606. [Abstract] [Full Text] [PDF]

				A. Rigotti, H. E. Miettinen, and M. Krieger The Role of the High-Density Lipoprotein Receptor SR-BI in the Lipid Metabolism of Endocrine and Other Tissues Endocr. Rev., June 1, 2003; 24(3): 357 - 387. [Abstract] [Full Text] [PDF]

				P. Perez-Martinez, J. M Ordovas, J. Lopez-Miranda, P. Gomez, C. Marin, J. Moreno, F. Fuentes, R. A. Fernandez de la Puebla, and F. Perez-Jimenez Polymorphism exon 1 variant at the locus of the scavenger receptor class B type I gene: influence on plasma LDL cholesterol in healthy subjects during the consumption of diets with different fat contents Am. J. Clinical Nutrition, April 1, 2003; 77(4): 809 - 813. [Abstract] [Full Text] [PDF]

				M. C. de Beer, Z. Zhao, N. R. Webb, D. R. van der Westhuyzen, and W. J. S. de Villiers Lack of a direct role for macrosialin in oxidized LDL metabolism J. Lipid Res., April 1, 2003; 44(4): 674 - 685. [Abstract] [Full Text] [PDF]

				W. Hatahet, L. Cole, B. J. Kudchodkar, and T. V. Fungwe Dietary Fats Differentially Modulate the Expression of Lecithin:Cholesterol Acyltransferase, Apoprotein-A1 and Scavenger Receptor B1 in Rats J. Nutr., March 1, 2003; 133(3): 689 - 694. [Abstract] [Full Text] [PDF]

				S. Azhar, A. Nomoto, and E. Reaven Hormonal regulation of adrenal microvillar channel formation J. Lipid Res., June 1, 2002; 43(6): 861 - 871. [Abstract] [Full Text] [PDF]

				S. Munoz, M. Merlos, D. Zambon, C. Rodriguez, J. Sabate, E. Ros, and J. C. Laguna Walnut-enriched diet increases the association of LDL from hypercholesterolemic men with human HepG2 cells J. Lipid Res., December 1, 2001; 42(12): 2069 - 2076. [Abstract] [Full Text] [PDF]

				L. Zhou and A. Nilsson Sources of eicosanoid precursor fatty acid pools in tissues J. Lipid Res., October 1, 2001; 42(10): 1521 - 1542. [Abstract] [Full Text] [PDF]

				W. Khovidhunkit, A. H. Moser, J. K. Shigenaga, C. Grunfeld, and K. R. Feingold Regulation of scavenger receptor class B type I in hamster liver and Hep3B cells by endotoxin and cytokines J. Lipid Res., October 1, 2001; 42(10): 1636 - 1644. [Abstract] [Full Text] [PDF]

				K. W. Huggins, P. L. Colvin, E. R. Burleson, K. Kelley, J. K. Sawyer, P. H. R. Barrett, L. L. Rudel, and J. S. Parks Dietary n-3 polyunsaturated fat increases the fractional catabolic rate of medium-sized HDL particles in African green monkeys J. Lipid Res., September 1, 2001; 42(9): 1457 - 1466. [Abstract] [Full Text] [PDF]

				S. F. Cai, R. J. Kirby, P. N. Howles, and D. Y. Hui Differentiation-dependent expression and localization of the class B type I scavenger receptor in intestine J. Lipid Res., June 1, 2001; 42(6): 902 - 909. [Abstract] [Full Text]

				W. Witt, I. Kolleck, H. Fechner, P. Sinha, and B. Rüstow Regulation by vitamin E of the scavenger receptor BI in rat liver and HepG2 cells J. Lipid Res., December 1, 2000; 41(12): 2009 - 2016. [Abstract] [Full Text]

				J. Férézou, M. Combettes-Souverain, M. Souidi, J. L. Smith, N. Boehler, F. Milliat, E. Eckhardt, G. Blanchard, M. Riottot, C. Sérougne, et al. Cholesterol, bile acid, and lipoprotein metabolism in two strains of hamster, one resistant, the other sensitive (LPN) to sucrose-induced cholelithiasis J. Lipid Res., December 1, 2000; 41(12): 2042 - 2054. [Abstract] [Full Text]

				C. E. Walden, B. M. Retzlaff, B. L. Buck, S. Wallick, B. S. McCann, and R. H. Knopp Differential Effect of National Cholesterol Education Program (NCEP) Step II Diet on HDL Cholesterol, Its Subfractions, and Apoprotein A-I Levels in Hypercholesterolemic Women and Men After 1 Year : The beFIT Study Arterioscler. Thromb. Vasc. Biol., June 1, 2000; 20(6): 1580 - 1587. [Abstract] [Full Text] [PDF]

				A. Rigotti and M. Krieger Getting a Handle on "Good" Cholesterol with the High-Density Lipoprotein Receptor N. Engl. J. Med., December 23, 1999; 341(26): 2011 - 2013. [Full Text] [PDF]

				K. Alam, R. S. Meidell, and D. K. Spady Effect of Up-regulating Individual Steps in the Reverse Cholesterol Transport Pathway on Reverse Cholesterol Transport in Normolipidemic Mice J. Biol. Chem., May 4, 2001; 276(19): 15641 - 15649. [Abstract] [Full Text] [PDF]