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Correspondence to:
Jere P. Segrest
The process of assembly of apolipoprotein (apo) B-containing lipoprotein particles occurs co-translationally after disulfide-dependent folding of the N-terminal domain of apoB but the mechanism is not understood. During a recent database search for protein sequences that contained similar amphipathic ß strands to apoB-100, four vitellogenins, the precursor form of lipovitellin, an egg yolk lipoprotein, from chicken, frog, lamprey, and C. elegans appeared on the list of candidate proteins. The X-ray crystal structure of lamprey lipovitellin is known to contain a "lipid pocket" lined by antiparallel amphipathic ß sheets. Here we report that the first 1000 residues of human apoB-100 (the
Based upon these results, we suggest that an LV-like "proteolipid" intermediate containing a "lipid pocket" is formed by the N-terminal portion of apoB alone or, more likely, as a complex with MTP. This intermediate produces a lipid nidus required for assembly of apoB-containing lipoprotein particles; pocket expansion through the addition of amphipathic ß strands from the ß1 domain of apoB results in the formation of a progressively larger high density lipoprotein (HDL)-like, then very low density lipoprotein (VLDL)-like, spheroidal lipoprotein particle.—Segrest, J. P., M. K. Jones, and N. Dashti. N-terminal domain of apolipoprotein B has structural homology to lipovitellin and microsomal triglyceride transfer protein: a "lipid pocket" model for self-assembly of apoB-containing lipoprotein particles. J. Lipid Res. 1999. 40: 1401;–1416.
Supplementary key words:
plasma apolipoproteins, LOCATE, microsomal triglyceride transfer protein (MTP), amphipathic ß sheets, amphipathic
Copyright © 1999 by Lipid Research, Inc.
Original Article
N-terminal domain of apolipoprotein B has structural homology to lipovitellin and microsomal triglyceride transfer protein: a "lipid pocket" model for self-assembly of apoB-containing lipoprotein particles
Jere P. Segresta,
Martin K. Jonesa, and
Nassrin Dashtib
a Departments of Medicine and Biochemistry and the Atherosclerosis Research Unit, University of Alabama Medical Center, Birmingham, AL 35294-0012
b Departments of Nutrition Sciences and the Atherosclerosis Research Unit, University of Alabama Medical Center, Birmingham, AL 35294-0011
1 domain plus the first 200 residues of the ß1 domain) have sequence and amphipathic motif homologies to the lipid-binding pocket of lamprey lipovitellin. We also show that most of the 1 domain of human apoB-100 has sequence and amphipathic motif homologies to human microsomal triglyceride transfer protein (MTP), a protein required for assembly of apoB-containing lipoproteins. helixes, co-translational, vitellogenin, lipovitellin precursor, 1 domain, ß1 domain
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