|
 |
|
|||||||
|
|||||||||
Correspondence to:
Daniel J. Rader
In addition to its role in the uptake of apolipoprotein B (apoB)-containing lipoproteins, apoE promotes hepatic very low density lipoprotein-triglyceride (VLDL-TG) production in animal models. However, it is not known if apoE increases the amount of TG per VLDL particle or the number of VLDL particles secreted. VLDL-apoB production is a measure of the rate of VLDL particle secretion. We determined the effects of apoE deficiency and apoE overexpression on VLDL-apoB production in mice. [35S]methionine was injected into endogenously label VLDL-apoB and Triton WR-1339 was simultaneously injected to block the catabolism of VLDL. Compared with wild-type mice, the VLDL-apoB production rate was decreased by 33% in apoE-deficient mice. Conversely, VLDL-apoB production was increased by 48% in mice overexpressing apoE compared with controls. Nascent VLDL, obtained from post-Triton plasma, had a decreased, not increased, content of TG per apoB in the apoE-overexpressing group compared with the control group.
This study demonstrates that hepatic apoE expression increases the output of VLDL triglyceride by increasing the production rate of VLDL-apoB, suggesting that hepatic apoE influences the number of VLDL particles secreted by the liver.—Maugeais, C., U. J. F. Tietge, K. Tsukamoto, J. M. Glick, and D. J. Rader. Hepatic apolipoprotein E expression promotes very low density lipoprotein-apolipoprotein B production in vivo in mice. J. Lipid Res. 2000. 41: 1673;–1679.
Supplementary key words:
lipoproteins, recombinant adenovirus, metabolism
Copyright © 2000 by Lipid Research, Inc.
Original Article
Hepatic apolipoprotein E expression promotes very low density lipoprotein-apolipoprotein B production in vivo in mice
Cyrille Maugeaisa,
Uwe J. F. Tietgea,
Kazuhisa Tsukamotoa,
Jane M. Glickb, and
Daniel J. Radera
a Department of Medicine, University of Pennsylvania Health System, Philadelphia, PA 19104
b Department of Molecular and Cellular Engineering, University of Pennsylvania Health System, Philadelphia, PA 19104
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  P. Parini, U. Gustafsson, M. A. Davis, L. Larsson, C. Einarsson, M. Wilson, M. Rudling, H. Tomoda, S. Omura, S. Sahlin, et al. Cholesterol Synthesis Inhibition Elicits an Integrated Molecular Response in Human Livers Including Decreased ACAT2 Arterioscler Thromb Vasc Biol, June 1, 2008; 28(6): 1200 - 1206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Blasiole, A. T. Oler, and A. D. Attie Regulation of ApoB Secretion by the Low Density Lipoprotein Receptor Requires Exit from the Endoplasmic Reticulum and Interaction with ApoE or ApoB J. Biol. Chem., April 25, 2008; 283(17): 11374 - 11381. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Hofmann, D. Perez-Tilve, T. M. Greer, B. A. Coburn, E. Grant, J. E. Basford, M. H. Tschop, and D. Y. Hui Defective Lipid Delivery Modulates Glucose Tolerance and Metabolic Response to Diet in Apolipoprotein E Deficient Mice Diabetes, January 1, 2008; 57(1): 5 - 12. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wang, D. Gilham, and R. Lehner Proteomic and Lipid Characterization of Apolipoprotein B-free Luminal Lipid Droplets from Mouse Liver Microsomes: IMPLICATIONS FOR VERY LOW DENSITY LIPOPROTEIN ASSEMBLY J. Biol. Chem., November 9, 2007; 282(45): 33218 - 33226. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Gusarova, J. Seo, M. L. Sullivan, S. C. Watkins, J. L. Brodsky, and E. A. Fisher Golgi-associated Maturation of Very Low Density Lipoproteins Involves Conformational Changes in Apolipoprotein B, but Is Not Dependent on Apolipoprotein E J. Biol. Chem., July 6, 2007; 282(27): 19453 - 19462. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Westerterp, W. de Haan, J. F. P. Berbee, L. M. Havekes, and P. C. N. Rensen Endogenous apoC-I increases hyperlipidemia in apoE-knockout mice by stimulating VLDL production and inhibiting LPL J. Lipid Res., June 1, 2006; 47(6): 1203 - 1211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ikewaki, W. Cain, F. Thomas, R. Shamburek, L. A. Zech, D. Usher, H. B. Brewer Jr., and D. J. Rader Abnormal in vivo metabolism of apoB-containing lipoproteins in human apoE deficiency J. Lipid Res., July 1, 2004; 45(7): 1302 - 1311. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Ng, C. Xie, G. F. Maguire, X. Zhu, F. Ugwu, E. Lam, and P. W. Connelly Hypertriglyceridemia in Lecithin-cholesterol Acyltransferase-deficient Mice Is Associated with Hepatic Overproduction of Triglycerides, Increased Lipogenesis, and Improved Glucose Tolerance J. Biol. Chem., February 27, 2004; 279(9): 7636 - 7642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. P. Newberry, Y. Xie, S. Kennedy, X. Han, K. K. Buhman, J. Luo, R. W. Gross, and N. O. Davidson Decreased Hepatic Triglyceride Accumulation and Altered Fatty Acid Uptake in Mice with Deletion of the Liver Fatty Acid-binding Protein Gene J. Biol. Chem., December 19, 2003; 278(51): 51664 - 51672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Raffai, A. H. Hasty, Y. Wang, S. E. Mettler, D. A. Sanan, M. F. Linton, S. Fazio, and K. H. Weisgraber Hepatocyte-derived ApoE Is More Effective than Non-hepatocyte-derived ApoE in Remnant Lipoprotein Clearance J. Biol. Chem., March 21, 2003; 278(13): 11670 - 11675. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Gerritsen, K. E. Kypreos, A. van der Zee, B. Teusink, V. I. Zannis, L. M. Havekes, and K. W. van Dijk Hyperlipidemia in APOE2 transgenic mice is ameliorated by a truncated apoE variant lacking the C-terminal domain J. Lipid Res., February 1, 2003; 44(2): 408 - 414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Cohn, M. Tremblay, R. Batal, H. Jacques, L. Veilleux, C. Rodriguez, P. H. R. Barrett, D. Dubreuil, M. Roy, L. Bernier, et al. Effect of atorvastatin on plasma apoE metabolism in patients with combined hyperlipidemia J. Lipid Res., September 1, 2002; 43(9): 1464 - 1471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Hinsdale, P. M. Sullivan, H. Mezdour, and N. Maeda ApoB-48 and apoB-100 differentially influence the expression of type-III hyperlipoproteinemia in APOE*2 mice J. Lipid Res., September 1, 2002; 43(9): 1520 - 1528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Millar, C. Maugeais, I. V. Fuki, and D. J. Rader Normal Production Rate of Apolipoprotein B in LDL Receptor-Deficient Mice Arterioscler Thromb Vasc Biol, June 1, 2002; 22(6): 989 - 994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Raffai and K. H. Weisgraber Hypomorphic Apolipoprotein E Mice. A NEW MODEL OF CONDITIONAL GENE REPAIR TO EXAMINE APOLIPOPROTEIN E-MEDIATED METABOLISM J. Biol. Chem., March 22, 2002; 277(13): 11064 - 11068. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Mensenkamp, B. Teusink, J. F.W. Baller, H. Wolters, R. Havinga, K. W. van Dijk, L. M. Havekes, and F. Kuipers Mice Expressing Only the Mutant APOE3Leiden Gene Show Impaired VLDL Secretion Arterioscler Thromb Vasc Biol, August 1, 2001; 21(8): 1366 - 1372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-a. Kawashiri, Y. Zhang, D. Usher, M. Reilly, E. Puré, and D. J. Rader Effects of coexpression of the LDL receptor and apoE on cholesterol metabolism and atherosclerosis in LDL receptor-deficient mice J. Lipid Res., June 1, 2001; 42(6): 943 - 950. [Abstract] [Full Text]
|
|
|
|
|
|
R. K. Tangirala, D. Pratico, G. A. FitzGerald, S. Chun, K. Tsukamoto, C. Maugeais, D. C. Usher, E. Pure, and D. J. Rader Reduction of Isoprostanes and Regression of Advanced Atherosclerosis by Apolipoprotein E J. Biol. Chem., January 5, 2001; 276(1): 261 - 266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Teusink, A. R. Mensenkamp, H. van der Boom, F. Kuipers, K. W. van Dijk, and L. M. Havekes Stimulation of the in Vivo Production of Very Low Density Lipoproteins by Apolipoprotein E Is Independent of the Presence of the Low Density Lipoprotein Receptor J. Biol. Chem., October 26, 2001; 276(44): 40693 - 40697. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |